Palmer, Kelli L.
Permanent URI for this collectionhttps://hdl.handle.net/10735.1/4295
Kelli Palmer is an Assistant Professor in the Department of Molecular and Cell Biology. Dr. Palmer uses genomic, transcriptomic, and biochemical approaches to study antibiotic resistance in pathogenic bacteria. Her research focuses on microorganisms contributing to significant mortality and cost burdens in the health care industry.
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Browsing Palmer, Kelli L. by Author "Frizzo, Clarissa P."
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Item Dicationic Imidazolium-Based Ionic Liquids: A New Strategy for Non-Toxic and Antimicrobial Materials(Royal Soc Chemistry) Gindri, Izabelle M.; Siddiqui, Danyal A.; Bhardwaj, Pooja; Rodriguez, Lucas C.; Palmer, Kelli L.; Frizzo, Clarissa P.; Martins, Marcos A. P.; Rodrigues, Danieli C.; Palmer, Kelli L.; Rodrigues, Danieli C.New dicationic imidazolium-based ionic liquids (ILs) were synthesized, characterized and tested in regards to cytotoxicity and antimicrobial activity. Insertion of a new cationic head and use of organic anions increased the biocompatibility of the ILs developed. IC₅₀ (concentration necessary to inhibit 50% of enzymatic activity) values obtained were considerably higher than those described for monocationic ILs, which indicates an improvement in cytocompatibility. Antimicrobial activity against bacterial species of clinical relevance in wounds and the oral environment was tested. The results showed that ILs were effective in inhibiting bacterial growth even below the minimum inhibitory concentration (MIC). It was observed that structural features that confer higher hydrophobicity to ILs decreased both the IC₅₀ and MIC simultaneously. However, it was possible to establish an equilibrium between those two effects, which gives the safe range of concentrations that ILs can be employed. The results demonstrated that the dicationic-imidazolium-based ILs synthesized may constitute a potent strategy for applications requiring non-toxic materials exhibiting antimicrobial activity.Item Evaluation of Mammalian and Bacterial Cell Activity on Titanium Surface Coated with Dicationic Imidazolium-Based Ionic Liquids(Royal Soc Chemistry, 2016-04-04) Gindri, Izabelle M.; Palmer, Kelli L.; Siddiqui, Danyal A.; Aghyarian, Shant; Frizzo, Clarissa P.; Martins, Marcos A. P.; Rodrigues, Danieli C.; 0000-0002-7343-9271 (Palmer, KL); Gindri, Izabelle M.; Palmer, Kelli L.; Siddiqui, Danyal A.; Aghyarian, Shant; Rodrigues, Danieli C.This work presents a new strategy to protect titanium surfaces against bacterial colonization and biofilm formation using dicationic imidazolium-based ionic liquid coatings. Ionic liquids (ILs) were designed as multi-functional coatings and their compatibility with human gingival fibroblasts (HGF-1) and preosteoblast (MC3T3-E1) cells was investigated. Results demonstrated that IL coatings were stable and present on titanium surfaces after 7 days of immersion and showed that using phenylalanine as the anionic moiety allowed for cell proliferation and differentiation on titanium surface while also providing strong antimicrobial and anti-biofilm activity against bacterial strains relevant to the oral environment (Streptococcus sp.). Strains such as Streptococcus mutans, S. sanguinis, S salivarius, S. gordonii and S. uberis are known to colonize the surface of dental implants in the early stages after implantation (early colonizers), compromising the success of these devices. The "race for the surface" between cells and bacteria was established by correlating results obtained from cell proliferation (epithelial and osteoblast) and differentiation (osteoblast) studies with that of antimicrobial activity against early bacterial colonizers.