Browsing by Author "Jacobs, Michael"
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Item An Electrochemical Sensor for the Detection of Antibiotic Contaminants in WaterJacobs, Michael; Nagaraj, V. J.; Mertz, T.; Selvam, Anjan Panneer; Ngo, T.; Prasad, ShaliniA nanochannel-based electrochemical sensor for the detection of trace amounts of erythromycin has been developed. The sensor is capable of specifically detecting erythromycin, at a sensitivity of 0.001 parts per trillion, in various water samples and has potential utility in the assessment of environmental water quality.Item Nanochannel-Based Electrochemical Sensor for the Detection of Pharmaceutical Contaminants in WaterNagaraj, V. J.; Jacobs, Michael; Vattipalli, K. M.; Annam, V. P.; Prasad, ShaliniEffective real-time monitoring is the key to understanding and tackling the issue of pharmaceutical contamination of water. This research demonstrates the utility of an alumina nanochannel-based electrochemical sensor platform for the detection of ibuprofen in water derived from various sources. Our results indicate that the sensor is highly sensitive with a limit of detection at 0.25 pg mL⁻¹. The novel sensor described here has potential for application as a simple, rapid, inexpensive and highly reliable method for real-time environmental water quality assessment.Item A Novel Approach for Electrical Tuning of Nano-Textured Zinc Oxide Surfaces for Ultra-Sensitive Troponin-t Detection(The Royal Society of Chemistry) Munje, Ruijuta D.; Jacobs, Michael; Muthukumar, S.; Quadri, Bilal; Shanmugam, Nandhinee Radha; Prasad, Shalini; 0000 0001 2765 4678 (Prasad, S); Munje, Ruijuta D.; Jacobs, Michael; Quadri, Bilal; Shanmugam, Nandhinee Radha; Prasad, ShaliniWe have developed a label-free, non-faradaic, electrochemical sensor for ultra-sensitive detection of a cardiac biomarker, troponin-T by utilizing the stoichiometric surface compositions of nanotextured zinc oxide (ZnO) thin films. In this study, we show how the performance of a nanotextured zinc oxide based non-faradaic biosensor is modulated by differences in the fabrication parameters of the metal oxide thin film as well as the choice of cross-linkers. Two cross-linking molecules, dithiobis succinimidyl propionate and 3-aminopropyl triethoxysilane, demonstrate significantly different binding chemistries with zinc oxide. The non-faradaic electrochemical behaviour of the sensor due to the two linkers is compared by analyzing the troponin-T dose response using electrochemical impedance spectroscopy (EIS). The sensor performance associated with both linkers is compared based on the dynamic range and limit of detection. The sensor utilizing zinc surface terminations demonstrated a wider dynamic range between the two linkers. This range extended from 26% to 54% in phosphate buffered saline and from 21% to 65% in human serum, for a concentration range from 10 fg mL⁻¹ to 1 ng mL⁻¹ of troponin-T. The limit of detection was found to be at 10 fg mL⁻¹ and has potential utility in the development of point-of-care (POC) diagnostics for cardiovascular diseases. Fluorescence quantification analysis was also performed to further validate the specificity of the linker binding to the ZnO films. An ultrasensitive troponin-T biosensor can be designed by leveraging the zinc termination based surface chemistry for selective protein immobilization.