Browsing by Author "Luzuriaga, Michael A."
Now showing 1 - 2 of 2
- Results Per Page
- Sort Options
Item Supramolecular and Biomacromolecular Enhancement of Metal-Free Magnetic Resonance Imaging Contrast Agents(Royal Society of Chemistry) Lee, Hamilton; Shahrivarkevishah, Arezoo; Lumata, Jenica L.; Luzuriaga, Michael A.; Hagge, Laurel M.; Benjamin, Candace E.; Brohlin, Olivia R.; Parish, Christopher R.; Firouzi, Hamid R.; Nielsen, Stephen O.; Lumata, Lloyd L.; Gassensmith, Jeremiah J.; 0000-0003-2176-925X (Hamilton, L); 0000-0002-5420-1954 (Shahrivarkevishah, A); 0000-0002-4650-3768 (Lumata, JL)); 0000-0001-6128-8800 (Luzuriaga, MA); 0000-0003-1064-6991 (Hagge, LM); 0000-0002-9211-718X (Benjamin, CE); 0000-0003-3226-6711 (Brohlin, OR); 0000-0003-4537-5992 (Firouzi, HR); 0000-0003-3390-3313 (Nielsen, SO); 0000-0002-3647-3753 (Lumata, LL); 0000-0001-6400-8106 (Gassensmith, JJ); Lee, Hamilton; Shahrivarkevishah, Arezoo; Lumata, Jenica L.; Luzuriaga, Michael A.; Hagge, Laurel M.; Benjamin, Candace E.; Brohlin, Olivia R.; Parish, Christopher R.; Firouzi, Hamid R.; Nielsen, Stephen O.; Lumata, Lloyd L.; Gassensmith, Jeremiah J.Many contrast agents for magnetic resonance imaging are based on gadolinium, however side effects limit their use in some patients. Organic radical contrast agents (ORCAs) are potential alternatives, but are reduced rapidly in physiological conditions and have low relaxivities as single molecule contrast agents. Herein, we use a supramolecular strategy where cucurbit[8]uril binds with nanomolar affinities to ORCAs and protects them against biological reductants to create a stable radical in vivo. We further overcame the weak contrast by conjugating this complex on the surface of a self-assembled biomacromolecule derived from the tobacco mosaic virus.Item ZIF-8 Degrades in Cell Media, Serum, and Some—but Not All—Common Laboratory Buffers(Taylor and Francis Ltd., 2019-05-11) Luzuriaga, Michael A.; Benjamin, Candace E.; Gaertner, Michael W.; Lee, Hamilton; Herbert, Fabian C.; Mallick, Snipta; Gassensmith, Jeremiah J.; 0000-0001-6128-8800 (Luzuriaga, MA); 0000-0002-9211-718X (Benjamin, CE); 0000-0003-2911-8160 (Gaertner, MW); 0000-0003-2176-925X (Lee, H); 0000-0002-0543-7151 (Herbert FC); 0000-0001-8635-2354 (Mallick, S); 0000-0001-6400-8106 (Gassensmith, JJ); Luzuriaga, Michael A.; Benjamin, Candace E.; Gaertner, Michael W.; Lee, Hamilton; Herbert, Fabian C.; Mallick, Snipta; Gassensmith, Jeremiah J.Drug delivery using metal-organic frameworks (MOF) has elicited interest in their biocompatibility; however, few studies have been conducted on their stability in common buffers, cell media, and blood proteins. In particular, the use of ZIF-8, a MOF interconnected by Zn and methylimidazole, has been frequently employed. In this study, we tested single crystals of ZIF-8 with common laboratory buffers, cell media, and serum, and noted several issues. Buffers containing phosphate and bicarbonate alter the appearance and composition of ZIF-8; however, these buffers do not appear to cause cargo to leak out even when the ZIF-8 itself is displaced by phosphates. On the other hand, serum dissolves ZIF-8, causing premature cargo release. Our results show that ZIF-8 undergoes surface chemistry changes that may affect the interpretation of cellular uptake and cargo release data. On the other hand, it provides a rational explanation as to how ZIF-8 neatly dissolves in vivo. ©2019 Informa UK Limited, trading as Taylor & Francis Group.