Browsing by Author "Mallick, Snipta"
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Item ZIF-8 Degrades in Cell Media, Serum, and Some—but Not All—Common Laboratory Buffers(Taylor and Francis Ltd., 2019-05-11) Luzuriaga, Michael A.; Benjamin, Candace E.; Gaertner, Michael W.; Lee, Hamilton; Herbert, Fabian C.; Mallick, Snipta; Gassensmith, Jeremiah J.; 0000-0001-6128-8800 (Luzuriaga, MA); 0000-0002-9211-718X (Benjamin, CE); 0000-0003-2911-8160 (Gaertner, MW); 0000-0003-2176-925X (Lee, H); 0000-0002-0543-7151 (Herbert FC); 0000-0001-8635-2354 (Mallick, S); 0000-0001-6400-8106 (Gassensmith, JJ); Luzuriaga, Michael A.; Benjamin, Candace E.; Gaertner, Michael W.; Lee, Hamilton; Herbert, Fabian C.; Mallick, Snipta; Gassensmith, Jeremiah J.Drug delivery using metal-organic frameworks (MOF) has elicited interest in their biocompatibility; however, few studies have been conducted on their stability in common buffers, cell media, and blood proteins. In particular, the use of ZIF-8, a MOF interconnected by Zn and methylimidazole, has been frequently employed. In this study, we tested single crystals of ZIF-8 with common laboratory buffers, cell media, and serum, and noted several issues. Buffers containing phosphate and bicarbonate alter the appearance and composition of ZIF-8; however, these buffers do not appear to cause cargo to leak out even when the ZIF-8 itself is displaced by phosphates. On the other hand, serum dissolves ZIF-8, causing premature cargo release. Our results show that ZIF-8 undergoes surface chemistry changes that may affect the interpretation of cellular uptake and cargo release data. On the other hand, it provides a rational explanation as to how ZIF-8 neatly dissolves in vivo. ©2019 Informa UK Limited, trading as Taylor & Francis Group.