BBS Staff and Student Research
Permanent URI for this collectionhttps://hdl.handle.net/10735.1/2869
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Browsing BBS Staff and Student Research by Subject "Adulthood"
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Item Leaky Expression of Channelrhodopsin-2 (ChR2) In Ai32 Mouse Lines(Public Library of Science) Prabhakar, Arthi; Vujovic, D.; Cui, L.; Olson, W.; Luo, W.; Prabhakar, ArthiOptogenetics enables the selective activation of genetically-targeted neuronal populations using light-sensitive ion channels. Genetic strategies using Cre-dependent mouse strains, especially the Ai32 line expressing Channelrhodopsin (ChR2)-EYFP fusion protein, have been a popular means to drive opsin expression in a cell-type specific manner. Here we report a low level of leaky ‘off-target’ (Cre-independent) ChR2-EYFP expression in Ai32/ Ai32 homozygous mice throughout the nervous system. This leaky off-target expression was characterized in multiple prevalent nervous system regions using anti-EYFP immunostaining. Expression of full-length ChR2-EYFP protein was confirmed using immunoprecipitation followed by Western blotting. Notably, light stimulation of these ChR2-EYFP expressing neurons in the spinal cord dorsal horn did not induce detectable photocurrents in juvenile 4-week old mice. Given the wide use of the Ai32 line by many labs, our results suggest researchers should be vigilant of possible off-target ChR2-EYFP expression in their region of interest, especially when generating Ai32/Ai32 homozygotes to drive high levels of ChR2-EYFP expression in adult mice.Item Motivations for Faking Orgasm and Orgasm Consistency Among Young Adult Women(Elsevier Ltd, 2019-06-04) Barnett, M D.; Maciel, Idalia V.; Van Vleet, S.; Marsden, A. D.,III; Maciel, Idalia V.Women fake orgasm for partner-focused reasons and self-focused reasons, the latter of which include elevating their own sexual arousal. The purpose of this study was to investigate the relationships between motivations for faking orgasm and orgasm consistency within the sexual activities of receiving oral sex and sexual intercourse among young adult women (N = 998). For both receiving oral sex and sexual intercourse, women who faked orgasm in order to elevate their own sexual arousal had greater orgasm consistency, whereas women who faked orgasm out of fear or insecurity had lower orgasm consistency. Overall, the results suggest that self-focused motivations for faking orgasm – particularly elevating arousal – are more closely associated with orgasm consistency than partner-focused motivations for faking orgasm. ©2019 Elsevier Ltd