Rodrigues, Danieli C.
Permanent URI for this collectionhttps://hdl.handle.net/10735.1/4062
Danieli Rodrigues is an Assistant Professor of Biomedical Engineering. Serving as a Research Engineer within the medical device industry, she developed test methods for performance verification and validation of new designs of hip and knee prostheses and related surgical instrumentation. Her graduate research focused on orthopedic biomaterials, primarily working on the characterization of corrosion and failure mechanisms of hip implants and development of acrylic two-solution bone cements for treatment of spinal compression fractures.
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Browsing Rodrigues, Danieli C. by Subject "Biofilms"
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Item Evaluation of Mammalian and Bacterial Cell Activity on Titanium Surface Coated with Dicationic Imidazolium-Based Ionic Liquids(Royal Soc Chemistry, 2016-04-04) Gindri, Izabelle M.; Palmer, Kelli L.; Siddiqui, Danyal A.; Aghyarian, Shant; Frizzo, Clarissa P.; Martins, Marcos A. P.; Rodrigues, Danieli C.; 0000-0002-7343-9271 (Palmer, KL); Gindri, Izabelle M.; Palmer, Kelli L.; Siddiqui, Danyal A.; Aghyarian, Shant; Rodrigues, Danieli C.This work presents a new strategy to protect titanium surfaces against bacterial colonization and biofilm formation using dicationic imidazolium-based ionic liquid coatings. Ionic liquids (ILs) were designed as multi-functional coatings and their compatibility with human gingival fibroblasts (HGF-1) and preosteoblast (MC3T3-E1) cells was investigated. Results demonstrated that IL coatings were stable and present on titanium surfaces after 7 days of immersion and showed that using phenylalanine as the anionic moiety allowed for cell proliferation and differentiation on titanium surface while also providing strong antimicrobial and anti-biofilm activity against bacterial strains relevant to the oral environment (Streptococcus sp.). Strains such as Streptococcus mutans, S. sanguinis, S salivarius, S. gordonii and S. uberis are known to colonize the surface of dental implants in the early stages after implantation (early colonizers), compromising the success of these devices. The "race for the surface" between cells and bacteria was established by correlating results obtained from cell proliferation (epithelial and osteoblast) and differentiation (osteoblast) studies with that of antimicrobial activity against early bacterial colonizers.Item Evaluation of Oral Microbial Corrosion on the Surface Degradation of Dental Implant Materials(Wiley, 2018-08-13) Siddiqui, Danyal A.; Guida, Lidia; Sridhar, Sathyanarayanan; Valderrama, Pilar; Wilson, Thomas G., Jr.; Rodrigues, Danieli C.; 0000-0002-0389-0833 (Rodrigues, DC); Rodrigues, Danieli C.; Siddiqui, Danyal A.; Guida, Lidia; Sridhar, SathyanarayananBackground: Titanium (Ti) dominates as the material of choice for dental implant systems. Recently, titanium-zirconium alloy (TiZr) and zirconia (ZrO₂) have emerged as alternative materials due to higher mechanical strength and lower corrosion susceptibility. Oral pathogenic bacteria can colonize Ti surfaces, leading to surface degradation, which has yet to be investigated on TiZr and ZrO₂. The aim of this study was to compare in vitro oral bacterial adhesion and subsequent surface degradation on commercial Ti, TiZr, and ZrO₂ implants. Methods: Ti, TiZr, and ZrO₂ implants with sandblasted, acid-etched (SLA) surfaces in addition to modified SLA-treated (modSLA) Ti implants (n = 3) were immersed for 30 consecutive days in Streptococcus polyculture. Post-immersion, adherent bacterial count was quantified. Optical microscopy was used to assess qualitative degradation and score Ti-based implants based on degree of surface damage while electrochemical testing quantified corrosion behavior. Analysis of variance followed by post-hoc Tukey test was used to statistically compare quantitative results (alpha = 0.05). Results: Ti-SLA, Ti-modSLA, and TiZr-SLA implants exhibited localized features characteristic of corrosion attack while ZrO₂-SLA implants experienced minimal changes in surface morphology as compared to non-immersed control. Corrosion features were more numerous on Ti-modSLA implants but smaller in size as compared with those on Ti-SLA and TiZr-SLA implants. No significant differences in corrosion resistance (polarization resistance and corrosion rate) were observed between Ti-SLA, Ti-modSLA, and TiZr-SLA implants. Conclusion: TiZr and ZrO₂ dental implant surfaces were not more susceptible to colonization and surface degradation by oral Streptococcus species than commercially pure Ti implants.