Now showing items 1-3 of 3

    • Assembly and Validation of Versatile Transcription Activator-Like Effector Libraries 

      Li, Yi; Ehrhardt, Kristina; Zhang, Michael Q.; Bleris, Leonidas (Nature Publishing Group, 2014-05-06)
      The ability to perturb individual genes in genome-wide experiments has been instrumental in unraveling cellular and disease properties. Here we introduce, describe the assembly, and demonstrate the use of comprehensive and ...
    • Exploiting the CRISPR/Cas9 PAM Constraint for Single-Nucleotide Resolution Interventions 

      Li, Yi; Mendiratta, Saurabh; Ehrhardt, Kristina; Kashyap, Neha; White, Michael A.; Bleris, Leonidas (2016-01-20)
      CRISPR/Cas9 is an enabling RNA-guided technology for genome targeting and engineering. An acute DNA binding constraint of the Cas9 protein is the Protospacer Adjacent Motif (PAM). Here we demonstrate that the PAM requirement ...
    • Mapping the Operational Landscape of MicroRNAs in Synthetic Gene Circuits. 

      Quarton, Tyler; Ehrhardt, Kristina; Lee, James; Kannan, Srijaa; Li, Yi; Ma, Lan; Bleris, Leonidas (Nature Partner Journals, 2018-10-22)
      MicroRNAs are a class of short, noncoding RNAs that are ubiquitous modulators of gene expression, with roles in development, homeostasis, and disease. Engineered microRNAs are now frequently used as regulatory modules in ...