Dodani, Sheel C.

Permanent URI for this collectionhttps://hdl.handle.net/10735.1/8764

Sheel Dodani is an Assistant Professor of Chemistry and Biochemistry. She also serves as the Director of the Dodani Lab. According to her CV, her research "is focused on developing a toolkit to identify the cellular sources, molecular targets, and signaling roles of biologically relevant anions from the chemical biology/bioinorganic perspective."

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    Discovery and Characterization of a Naturally Occurring, Turn-On Yellow Fluorescent Protein Sensor for Chloride
    (Amer Chemical Soc, 2019-01) Tutol, Jasmine N.; Peng, Weicheng; Dodani, Sheel C.; 0000-0003-0271-6080 (Dodani, SC); Tutol, Jasmine N.; Peng, Weicheng; Dodani, Sheel C.
    Fluorescent proteins have been extensively engineered and applied as optical indicators for chloride in a variety of biological contexts. Surprisingly, given the biodiversity of fluorescent proteins, a naturally occurring chloride sensor has not been reported to date. Here, we present the identification and spectroscopic characterization of the yellow fluorescent protein from the jellyfish Phialidium sp. (phiYFP), a rare example of a naturally occurring, excitation ratiometric, and turn-on fluorescent protein sensor for chloride. Our results show that chloride binding tunes the pKₐ of the chromophore Y66 and shifts the equilibrium from the fluorescent phenolate form to the weakly fluorescent phenol form. The latter likely undergoes excited-state proton transfer to generate a turn-on fluorescence response that is pH-dependent. Moreover, anion selectivity and mutagenesis in the chloride binding pocket provide additional evidence for the proposed chloride sensing mechanism. Given these properties, we anticipate that phiYFP, with further engineering, could be a new tool for imaging cellular chloride dynamics.

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