Raimund Ober serves as a professor in the departments of Electrical Engineering and of Biomedical Engineering. He is also an adjunct professor in the Department of Immunology at the University of Texas Southwestern Medical Center and is co-director of the Ward Ober Lab there. Dr. Ober's "research is directed towards taking an interdisciplinary approach to generate effective therapeutics for autoimmunity and cancer. This involves a combination of antibody/protein engineering, fluorescence microscopy and in vivo studies in mice."
(Optical Society of American (OSA)) Tahmasbi, Amir; Ram, Sripad; Chao, Jerry; Abraham, Anish V.; Tang, Felix W.; Ward, E. S.; Ober, Raimund J.; 279877162 (Ober, RJ)
Multifocal plane microscopy (MUM) has made it possible to study subcellular dynamics in 3D at high temporal and spatial resolution by simultaneously imaging distinct planes within the specimen. MUM allows high accuracy localization of a point source along the z-axis since it overcomes the depth discrimination problem of conventional single plane microscopy. An important question in MUM experiments is how the number of focal planes and their spacings should be chosen to achieve the best possible localization accuracy along the z-axis. Here, we propose approaches based on the Fisher information matrix and report spacing scenarios called strong coupling and weak coupling which yield an appropriate 3D localization accuracy. We examine the effect of numerical aperture, magnification, photon count, emission wavelength and extraneous noise on the spacing scenarios. In addition, we investigate the effect of changing the number of focal planes on the 3D localization accuracy. We also introduce a new software package that provides a user-friendly framework to find appropriate plane spacings for a MUM setup. These developments should assist in optimizing MUM experiments.