Now showing items 1-3 of 3

    • DNA Looping in Topologically Constrained Domains 

      Bharadwaj, Anusha; Kesinger, Matthew R.; Shoura, Massa J.; Vetcher, Alexandre; Levene, Stephen D. (Oxford University Press., 2012-05-15)
      New techniques are needed in order to study the effects of DNA supercoiling on loop-mediated regulation. The thermodynamics of DNA looping depend periodically on the helical phasing between recognition sites. A long-term ...
    • Engineering Multiple Site-Specific Modifications in Supercoiled DNAs 

      Bharadwaj, Anusha; Kesinger, Matthew R.; Shoura, Massa J.; Vetcher, Alexandre; Levene, Stephen D. (2012-06-19)
      New techniques are needed in order to study the effects of DNA supercoiling on loop-mediated regulation. The thermodynamics of DNA looping depend periodically on the helical phasing between recognition sites. A long-term ...
    • Measurements of DNA-Loop Formation via Cre-Mediated Recombination 

      Shoura, Massa J.; Vetcher, Alexandre; Giovan, Stefan; Levene, Stephen D.; Bardai, Farah; Bharadwaj, Anusha; Kesinger, Matthew R. (2012-05-15)
      The Cre recombination system has become an important tool for genetic manipulation of higher organisms and a model for site-specific DNA-recombination mechanisms employed by the λ-Int superfamily of recombinases. We report ...