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    Exploiting the CRISPR/Cas9 PAM Constraint for Single-Nucleotide Resolution Interventions 

    Li, Yi; Mendiratta, Saurabh; Ehrhardt, Kristina; Kashyap, Neha; White, Michael A.; Bleris, Leonidas (2016-01-20)
    CRISPR/Cas9 is an enabling RNA-guided technology for genome targeting and engineering. An acute DNA binding constraint of the Cas9 protein is the Protospacer Adjacent Motif (PAM). Here we demonstrate that the PAM requirement ...
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    Transcripts for Combined Synthetic MicroRNA and Gene Delivery 

    Kashyap, Neha; Pham, Bich; Xie, Zhen; Bleris, Leonidas (2013-06-26)
    MicroRNAs (miRNAs) are a class of short noncoding RNAs which are endogenously expressed in many organisms and regulate gene expression by binding to messenger RNA (mRNA). MicroRNAs are either produced from their independent ...

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    AuthorBleris, Leonidas (2)Kashyap, Neha (2)Ehrhardt, Kristina (1)Li, Yi (1)Mendiratta, Saurabh (1)Pham, Bich (1)White, Michael A. (1)Xie, Zhen (1)Subject
    Plasmids (2)
    Cas9 protein, Francisella novicida (1)Codon (1)DNA (1)DsRed (1)Flow Cytometry (1)KRAS protein, human (1)MicroRNAs (1)Mutation (1)RNA, Guide (1)... View MoreDate Issued2013 (1)2016 (1)Has File(s)Yes (2)

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