Now showing items 1-4 of 4

    • Assembly and Validation of Versatile Transcription Activator-Like Effector Libraries 

      Li, Yi; Ehrhardt, Kristina; Zhang, Michael Q.; Bleris, Leonidas (Nature Publishing Group, 2014-05-06)
      The ability to perturb individual genes in genome-wide experiments has been instrumental in unraveling cellular and disease properties. Here we introduce, describe the assembly, and demonstrate the use of comprehensive and ...
    • CRISPR-Based Self-Cleaving Mechanism for Controllable Gene Delivery in Human Cells 

      Moore, Richard; Spinhirne, Alec; Lai, Michael J.; Preisser, Samantha; Li, Yi; Kang, Taek; Bleris, Leonidas (Oxford University Press, 2014-12-18)
      Controllable gene delivery via vector-based systems remains a formidable challenge in mammalian synthetic biology and a desirable asset in gene therapy applications. Here, we introduce a methodology to control the copies ...
    • Exploiting the CRISPR/Cas9 PAM Constraint for Single-Nucleotide Resolution Interventions 

      Li, Yi; Mendiratta, Saurabh; Ehrhardt, Kristina; Kashyap, Neha; White, Michael A.; Bleris, Leonidas (2016-01-20)
      CRISPR/Cas9 is an enabling RNA-guided technology for genome targeting and engineering. An acute DNA binding constraint of the Cas9 protein is the Protospacer Adjacent Motif (PAM). Here we demonstrate that the PAM requirement ...
    • Synthetic Mammalian Transgene Negative Autoregulation 

      Shimoga, Vinay; White, Jacob T.; Li, Yi; Sontag, Eduardo; Bleris, Leonidas (2013-06-04)
      Biological networks contain overrepresented small-scale topologies, typically called motifs. A frequently appearing motif is the transcriptional negative-feedback loop, where a gene product represses its own transcription. ...