Now showing items 1-2 of 2

    • Exploiting the CRISPR/Cas9 PAM Constraint for Single-Nucleotide Resolution Interventions 

      Li, Yi; Mendiratta, Saurabh; Ehrhardt, Kristina; Kashyap, Neha; White, Michael A.; Bleris, Leonidas (2016-01-20)
      CRISPR/Cas9 is an enabling RNA-guided technology for genome targeting and engineering. An acute DNA binding constraint of the Cas9 protein is the Protospacer Adjacent Motif (PAM). Here we demonstrate that the PAM requirement ...
    • Transcripts for Combined Synthetic MicroRNA and Gene Delivery 

      Kashyap, Neha; Pham, Bich; Xie, Zhen; Bleris, Leonidas (2013-06-26)
      MicroRNAs (miRNAs) are a class of short noncoding RNAs which are endogenously expressed in many organisms and regulate gene expression by binding to messenger RNA (mRNA). MicroRNAs are either produced from their independent ...