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dc.contributor.authorLi, Yien_US
dc.contributor.authorEhrhardt, Kristinaen_US
dc.contributor.authorZhang, Michael Q.en_US
dc.contributor.authorBleris, Leonidasen_US
dc.date.accessioned2014-09-12T21:37:58Z
dc.date.available2014-09-12T21:37:58Z
dc.date.created2014-05-06en_US
dc.date.issued2014-05-06en_US
dc.identifier.issn2045-2322en_US
dc.identifier.urihttp://hdl.handle.net/10735.1/4013
dc.descriptionIncludes supplementary material.en_US
dc.description.abstractThe ability to perturb individual genes in genome-wide experiments has been instrumental in unraveling cellular and disease properties. Here we introduce, describe the assembly, and demonstrate the use of comprehensive and versatile transcription activator-like effector (TALE) libraries. As a proof of principle, we built an 11-mer library that covers all possible combinations of the nucleotides that determine the TALE-DNA binding specificity. We demonstrate the versatility of the methodology by constructing a constraint library, customized to bind to a known p53 motif. To verify the functionality in assays, we applied the 11-mer library in yeast-one-hybrid screens to discover TALEs that activate human SCN9A and miR-34b respectively. Additionally, we performed a genome-wide screen using the complete 11-mer library to confirm known genes that confer cycloheximide resistance in yeast. Considering the highly modular nature of TALEs and the versatility and ease of constructing these libraries we envision broad implications for high-throughput genomic assays. ;en_US
dc.description.sponsorshipUS National Institutes of Health (GM098984, GM096271, CA17001801); US National Science Foundation (CBNET-1105524)en_US
dc.publisherNature Publishing Groupen_US
dc.relation.urihttp://dx.doi.org/10.1038/srep04857
dc.rightsCC BY-NC-SA 3.0 (Attribution-NonCommercial-ShareAlike)en_US
dc.rights©2014 Macmillan Publishers Ltd,en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/en_US
dc.sourceScientific Reports
dc.subjectTranscription Activator-Like Effector libraries (TALE)en_US
dc.subjectDNAen_US
dc.subjectMultidrug resistanceen_US
dc.subjectBait sequencesen_US
dc.titleAssembly and Validation of Versatile Transcription Activator-Like Effector Librariesen_US
dc.type.genrearticleen_US
dc.identifier.bibliographicCitationLi, Yi, Kristina Ehrhardt, Michael Q. Zhang, and Leonidas Bleris. 2014. "Assembly and validation of versatile transcription activator-like effector libraries." Scientific Reports 4(4857): 4857-4857.en_US
dc.identifier.volume4en_US
dc.identifier.issue4857en_US
dc.contributor.utdAuthorZhang, Michael Q.en_US
dc.contributor.ISNI0000 0001 2535 9739 (Bleris, L)
dc.contributor.ISNI0000 0001 1707 1372 (Zhang, MQ)
dc.contributor.LCNA2012076942‏ (Bleris, L)
dc.contributor.LCNA99086074‏ ‎(Zhang, MQ)


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CC BY-NC-SA 3.0 (Attribution-NonCommercial-ShareAlike)
Except where otherwise noted, this item's license is described as CC BY-NC-SA 3.0 (Attribution-NonCommercial-ShareAlike)