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dc.contributor.authorTahmasbi, Amiren_US
dc.contributor.authorRam, Sripaden_US
dc.contributor.authorChao, Jerryen_US
dc.contributor.authorAbraham, Anish V.en_US
dc.contributor.authorTang, Felix W.en_US
dc.contributor.authorWard, E. S.en_US
dc.contributor.authorOber, Raimund J.en_US
dc.date.accessioned2014-11-13T22:22:46Z
dc.date.available2014-11-13T22:22:46Z
dc.date.created2014-06-30
dc.identifier.issn1094-4087en_US
dc.identifier.urihttp://hdl.handle.net/10735.1/4173
dc.description.abstractMultifocal plane microscopy (MUM) has made it possible to study subcellular dynamics in 3D at high temporal and spatial resolution by simultaneously imaging distinct planes within the specimen. MUM allows high accuracy localization of a point source along the z-axis since it overcomes the depth discrimination problem of conventional single plane microscopy. An important question in MUM experiments is how the number of focal planes and their spacings should be chosen to achieve the best possible localization accuracy along the z-axis. Here, we propose approaches based on the Fisher information matrix and report spacing scenarios called strong coupling and weak coupling which yield an appropriate 3D localization accuracy. We examine the effect of numerical aperture, magnification, photon count, emission wavelength and extraneous noise on the spacing scenarios. In addition, we investigate the effect of changing the number of focal planes on the 3D localization accuracy. We also introduce a new software package that provides a user-friendly framework to find appropriate plane spacings for a MUM setup. These developments should assist in optimizing MUM experiments.en_US
dc.description.sponsorshipUS National Institutes of Health (R01 GM085575)en_US
dc.language.isoenen_US
dc.publisherOptical Society of American (OSA)en_US
dc.relation.urihttp://dx.doi.org/10.1364/OE.22.016706en_US
dc.rights©2014 Optical Society of Americaen_US
dc.subjectMultifocal plane microscopyen_US
dc.subjectPractical localization accuracy measureen_US
dc.subjectFocal planesen_US
dc.titleDesigning the Focal Plane Spacing for Multifocal Plane Microscopyen_US
dc.type.genrearticleen_US
dc.identifier.bibliographicCitationTahmasbi, A., S. Ram, J. Chao, A. V. Abraham, et al. 2014. "Designing the focal plane spacing for multifocal plane microscopy." Optics Express 22(14): 16706-16721.
dc.source.journalOptics Expressen_US
dc.identifier.volume22en_US
dc.identifier.issue14en_US
dc.identifier.startpage16706en_US
dc.contributor.VIAF279877162 (Ober, RJ)en_US


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