Show simple item record

dc.contributor.authorPadavannil, A.
dc.contributor.authorSarkar, Prithwijit
dc.contributor.authorKim, S. J.
dc.contributor.authorCagatay, T.
dc.contributor.authorJiou, J.
dc.contributor.authorBrautigam, C. A.
dc.contributor.authorTomchick, D. R.
dc.contributor.authorSali, A.
dc.contributor.authorD'Arcy, Sheena
dc.contributor.authorChook, Y. M.
dc.date.accessioned2019-11-18T21:44:44Z
dc.date.available2019-11-18T21:44:44Z
dc.date.created2019
dc.identifier.issn2050-084X
dc.identifier.urihttps://hdl.handle.net/10735.1/7126
dc.description.abstractWe report the crystal structure of nuclear import receptor Importin-9 bound to its cargo, the histones H2A-H2B. Importin-9 wraps around the core, globular region of H2A-H2B to form an extensive interface. The nature of this interface coupled with quantitative analysis of deletion mutants of H2A-H2B suggests that the NLS-like sequences in the H2A-H2B tails play a minor role in import. Importin-9•H2A-H2B is reminiscent of interactions between histones and histone chaperones in that it precludes H2A-H2B interactions with DNA and H3-H4 as seen in the nucleosome. Like many histone chaperones, which prevent inappropriate non-nucleosomal interactions, Importin-9 also sequesters H2A-H2B from DNA. Importin-9 appears to act as a storage chaperone for H2A-H2B while escorting it to the nucleus. Surprisingly, RanGTP does not dissociate Importin-9•H2A-H2B but assembles into a RanGTP•Importin-9•H2A-H2B complex. The presence of Ran in the complex, however, modulates Imp9-H2A-H2B interactions to facilitate its dissociation by DNA and assembly into a nucleosome. © 2019 Padavannil et al.
dc.description.sponsorshipNational Institute of General Medical Sciences (U01GM98256-01, R01GM083960, P41GM109824, R01GM112108, R01GM069909); Welch Foundation (I-1532).
dc.language.isoen
dc.publisherNLM (Medline)
dc.relation.urihttps://dx.doi.org/10.7554/eLife.43630
dc.rightsCC BY 4.0 (Attribution)
dc.rights©2019 The Authors
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectEscherichia coli
dc.subjectHistones
dc.subjectHistone Chaperones
dc.subjectImportin-9
dc.subjectKaryopherins
dc.subjectMolecular biology
dc.subjectNucleosomes
dc.subjectBiology, Structural
dc.titleImportin-9 Wraps Around the H2A-H2B Core to Act as Nuclear Importer and Histone Chaperone
dc.type.genrearticle
dc.description.departmentSchool of Natural Sciences and Mathematics
dc.identifier.bibliographicCitationPadavannil, A., P. Sarkar, S. J. Kim, T. Cagatay, et al. 2019. "Importin-9 wraps around the H2A-H2B core to act as nuclear importer and histone chaperone." eLife 8.
dc.source.journaleLife
dc.identifier.volume8
dc.contributor.utdAuthorSarkar, Prithwijit
dc.contributor.utdAuthorD'Arcy, Sheena
dc.contributor.ORCID0000-0001-5055-988X (D'Arcy, S)


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

CC BY 4.0 (Attribution)
Except where otherwise noted, this item's license is described as CC BY 4.0 (Attribution)