Discovery and Characterization of a Naturally Occurring, Turn-On Yellow Fluorescent Protein Sensor for Chloride

dc.contributor.ORCID0000-0003-0271-6080 (Dodani, SC)
dc.contributor.authorTutol, Jasmine N.
dc.contributor.authorPeng, Weicheng
dc.contributor.authorDodani, Sheel C.
dc.contributor.utdAuthorTutol, Jasmine N.
dc.contributor.utdAuthorPeng, Weicheng
dc.contributor.utdAuthorDodani, Sheel C.
dc.date.accessioned2020-08-03T13:48:02Z
dc.date.available2020-08-03T13:48:02Z
dc.date.issued2019-01
dc.descriptionDue to copyright restrictions and/or publisher's policy full text access from Treasures at UT Dallas is limited to current UTD affiliates (use the provided Link to Article).
dc.descriptionSupplementary material is available on publisher's website. Use the DOI link below.
dc.description.abstractFluorescent proteins have been extensively engineered and applied as optical indicators for chloride in a variety of biological contexts. Surprisingly, given the biodiversity of fluorescent proteins, a naturally occurring chloride sensor has not been reported to date. Here, we present the identification and spectroscopic characterization of the yellow fluorescent protein from the jellyfish Phialidium sp. (phiYFP), a rare example of a naturally occurring, excitation ratiometric, and turn-on fluorescent protein sensor for chloride. Our results show that chloride binding tunes the pKₐ of the chromophore Y66 and shifts the equilibrium from the fluorescent phenolate form to the weakly fluorescent phenol form. The latter likely undergoes excited-state proton transfer to generate a turn-on fluorescence response that is pH-dependent. Moreover, anion selectivity and mutagenesis in the chloride binding pocket provide additional evidence for the proposed chloride sensing mechanism. Given these properties, we anticipate that phiYFP, with further engineering, could be a new tool for imaging cellular chloride dynamics.
dc.description.departmentSchool of Natural Sciences and Mathematics
dc.description.sponsorshipWelch Foundation (AT-1918- 20170325), National Institutes of Health (1R35GM128923-01)
dc.identifier.bibliographicCitationTutol, Jasmine N., Weicheng Peng, and Sheel C. Dodani. 2019. "Discovery and Characterization of a Naturally Occurring, Turn-On Yellow Fluorescent Protein Sensor for Chloride." Biochemistry 58(1): 31-35, doi: 10.1021/acs.biochem.8b00928
dc.identifier.issn0006-2960
dc.identifier.issue1
dc.identifier.urihttp://dx.doi.org/10.1021/acs.biochem.8b00928
dc.identifier.urihttps://hdl.handle.net/10735.1/8765
dc.identifier.volume58
dc.language.isoen
dc.publisherAmer Chemical Soc
dc.rights©2018 American Chemical Society
dc.source.journalBiochemistry
dc.subjectHydrogen-ion concentration
dc.subjectChloride channels
dc.subjectProtons
dc.subjectBiochemistry
dc.subjectMolecular biology
dc.subjectYellow fluorescent protein
dc.titleDiscovery and Characterization of a Naturally Occurring, Turn-On Yellow Fluorescent Protein Sensor for Chloride
dc.type.genrearticle

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