A Novel Approach for Electrical Tuning of Nano-Textured Zinc Oxide Surfaces for Ultra-Sensitive Troponin-t Detection

dc.contributor.ISNI0000 0001 2765 4678 (Prasad, S)en_US
dc.contributor.authorMunje, Ruijuta D.en_US
dc.contributor.authorJacobs, Michaelen_US
dc.contributor.authorMuthukumar, S.en_US
dc.contributor.authorQuadri, Bilalen_US
dc.contributor.authorShanmugam, Nandhinee Radhaen_US
dc.contributor.authorPrasad, Shalinien_US
dc.contributor.utdAuthorMunje, Ruijuta D.
dc.contributor.utdAuthorJacobs, Michael
dc.contributor.utdAuthorQuadri, Bilal
dc.contributor.utdAuthorShanmugam, Nandhinee Radha
dc.contributor.utdAuthorPrasad, Shalini
dc.date.accessioned2016-03-28T19:03:36Z
dc.date.available2016-03-28T19:03:36Z
dc.date.created2015-12-21
dc.description.abstractWe have developed a label-free, non-faradaic, electrochemical sensor for ultra-sensitive detection of a cardiac biomarker, troponin-T by utilizing the stoichiometric surface compositions of nanotextured zinc oxide (ZnO) thin films. In this study, we show how the performance of a nanotextured zinc oxide based non-faradaic biosensor is modulated by differences in the fabrication parameters of the metal oxide thin film as well as the choice of cross-linkers. Two cross-linking molecules, dithiobis succinimidyl propionate and 3-aminopropyl triethoxysilane, demonstrate significantly different binding chemistries with zinc oxide. The non-faradaic electrochemical behaviour of the sensor due to the two linkers is compared by analyzing the troponin-T dose response using electrochemical impedance spectroscopy (EIS). The sensor performance associated with both linkers is compared based on the dynamic range and limit of detection. The sensor utilizing zinc surface terminations demonstrated a wider dynamic range between the two linkers. This range extended from 26% to 54% in phosphate buffered saline and from 21% to 65% in human serum, for a concentration range from 10 fg mL⁻¹ to 1 ng mL⁻¹ of troponin-T. The limit of detection was found to be at 10 fg mL⁻¹ and has potential utility in the development of point-of-care (POC) diagnostics for cardiovascular diseases. Fluorescence quantification analysis was also performed to further validate the specificity of the linker binding to the ZnO films. An ultrasensitive troponin-T biosensor can be designed by leveraging the zinc termination based surface chemistry for selective protein immobilization.en_US
dc.identifier.bibliographicCitationMunje, R. D., M. Jacobs, S. Muthukumar, B. Quadri, N. R. Shanmugam, et al. 2015. "A novel approach for electrical tuning of nano-textured zinc oxide surfaces for ultra-sensitive troponin-T detection." Analytical Methods 7(24): 10136-10144.en_US
dc.identifier.issn1759-9660en_US
dc.identifier.issue24en_US
dc.identifier.urihttp://hdl.handle.net/10735.1/4809
dc.identifier.volume7en_US
dc.publisherThe Royal Society of Chemistryen_US
dc.relation.urihttp://dx.doi.org/10.1039/c5ay02052ben_US
dc.rights©2015 The Royal Society of Chemistry. This article may not be further made available or distributed.en_US
dc.source.journalAnalytical Methodsen_US
dc.subjectBiosensorsen_US
dc.subjectImpedance spectroscopyen_US
dc.subjectElectrochemical sensorsen_US
dc.subjectSurface chemistryen_US
dc.subjectThin filmsen_US
dc.subjectZinc oxideen_US
dc.subjectCardiovascular Diseasesen_US
dc.subjectdithiobis(succinimidylpropionate)en_US
dc.subjectPhosphate-buffered salineen_US
dc.subjectImmobilized proteinsen_US
dc.titleA Novel Approach for Electrical Tuning of Nano-Textured Zinc Oxide Surfaces for Ultra-Sensitive Troponin-t Detectionen_US
dc.type.genrearticleen_US

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