Examining the Effect of Microbial Community on CRISPR-Cas Activity in Enterococcus Faecalis

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dc.contributor.advisorPalmer, Kelli
dc.contributor.committeeMemberDillon, Nicholas
dc.contributor.committeeMemberDe Nisco, Nicole
dc.creatorByregowda, Shrinidhi 1998-
dc.date.accessioned2023-10-13T21:10:00Z
dc.date.available2023-10-13T21:10:00Z
dc.date.created2023-05
dc.date.issuedMay 2023
dc.date.submittedMay 2023
dc.date.updated2023-10-13T21:10:00Z
dc.description.abstractEnterococcus faecalis is an opportunistic pathogen involved in causing various hospital-acquired infections. Their propensity to develop antibiotic resistance by horizontal gene transfer via conjugative mobile genetic elements makes the treatment of E. faecalis infection increasingly difficult. Pheromone-responsive plasmids are involved in the transfer of mobile genetic elements in E. faecalis. CRISPR-Cas acts as a barrier against foreign genome transfer in bacteria. E. faecalis CRISPR-Cas activity varies in vivo (murine intestinal model) and in vitro. The functional differences may be due to various biotic and abiotic factors such as nutrient availability, community diversity, donor-to-recipient ratios, and cas9 regulatory differences. A previous study has shown that the efficacy of CRISPR-Cas varies in the presence of a microbial community. Here we study the effect of Escherichia coli, also a resident of the mammalian gut, on CRISPR-Cas defense in vitro using E. faecalis containing pAM714 plasmid. Through our work, we are able to demonstrate that CRISPR-Cas efficacy is not altered by the presence of E. coli during conjugation between donor OG1SSp (pAM714) and recipients T11RF and T11RFΔcas9. Our goal is to provide insights into CRISPR-Cas function of Gram-positive bacteria in the microbial community.
dc.format.mimetypeapplication/pdf
dc.identifier.uri
dc.identifier.urihttps://hdl.handle.net/10735.1/9934
dc.language.isoEnglish
dc.subjectBiology, Microbiology
dc.titleExamining the Effect of Microbial Community on CRISPR-Cas Activity in Enterococcus Faecalis
dc.typeThesis
dc.type.materialtext
thesis.degree.collegeSchool of Natural Sciences and Mathematics
thesis.degree.departmentBiotechnology
thesis.degree.grantorThe University of Texas at Dallas
thesis.degree.nameMaster of Biotechnology

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