The Effect of Microfluidic Geometry on Myoblast Migration

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dc.contributor.ORCID0000-0002-9325-547X (Atmaramani, R)
dc.contributor.ORCID0000-0001-8276-3690 (Pancrazio, JJ)
dc.contributor.ORCID0000-0001-6404-318X (Schmidtke, DW)
dc.contributor.authorAtmaramani, Rahul
dc.contributor.authorBlack, Brian J.
dc.contributor.authorLam, Kevin H.
dc.contributor.authorSheth, Vinit M.
dc.contributor.authorPancrazio, Joseph J.
dc.contributor.authorSchmidtke, David W.
dc.contributor.authorAlsmadi, Nesreen Zoghoul
dc.contributor.utdAuthorAtmaramani, Rahul
dc.contributor.utdAuthorBlack, Brian J.
dc.contributor.utdAuthorLam, Kevin H.
dc.contributor.utdAuthorSheth, Vinit M.
dc.contributor.utdAuthorPancrazio, Joseph J.
dc.contributor.utdAuthorSchmidtke, David W.
dc.contributor.utdAuthorAlsmadi, Nesreen Zoghoul
dc.date.accessioned2019-10-31T15:12:15Z
dc.date.available2019-10-31T15:12:15Z
dc.date.created2019-02-21
dc.description.abstractIn vitro systems comprised of wells interconnected by microchannels have emerged as a platform for the study of cell migration or multicellular models. In the present study, we systematically evaluated the effect of microchannel width on spontaneous myoblast migration across these microchannels-from the proximal to the distal chamber. Myoblast migration was examined in microfluidic devices with varying microchannel widths of 1.5-20 µm, and in chips with uniform microchannel widths over time spans that are relevant for myoblast-to-myofiber differentiation in vitro. We found that the likelihood of spontaneous myoblast migration was microchannel width dependent and that a width of 3 µm was necessary to limit spontaneous migration below 5% of cells in the seeded well after 48 h. These results inform the future design of Polydimethylsiloxane (PDMS) microchannel-based co-culture platforms as well as future in vitro studies of myoblast migration. © 2019 by the authors.
dc.description.departmentErik Jonsson School of Engineering and Computer Science
dc.identifier.bibliographicCitationAtmaramani, R., B. J. Black, K. H. Lam, V. M. Sheth, et al. 2019. "The effect of microfluidic geometry on myoblast migration." Micromachines 10(2): art. 143, doi: 10.3390/mi10020143
dc.identifier.issn2072-666X
dc.identifier.issue2
dc.identifier.urihttps://hdl.handle.net/10735.1/7053
dc.identifier.volume10
dc.language.isoen
dc.publisherMDPI AG
dc.relation.urihttps://dx.doi.org/10.3390/mi10020143
dc.rightsCC BY 4.0 (Attribution)
dc.rights©2019 The Authors
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.source.journalMicromachines
dc.subjectMicrofabrication
dc.subjectMicrofluidics
dc.subjectMyoblasts
dc.subjectPolydimethylsiloxane
dc.subjectPulse-duration modulation
dc.subjectSilicone
dc.subjectCell migration
dc.titleThe Effect of Microfluidic Geometry on Myoblast Migration
dc.type.genrearticle

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Schmidtke, David W.
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