Characterization of C-Ring Component Assembly in Flagellar Motors from Amino Acid Coevolution

dc.contributor.authordos Santos, Ricardo Nascimento
dc.contributor.authorKhan, Shahid
dc.contributor.authorMorcos, Faruck
dc.contributor.utdAuthorMorcos, Faruck
dc.date.accessioned2019-05-16T20:58:35Z
dc.date.available2019-05-16T20:58:35Z
dc.date.created2018-05-09
dc.descriptionIncludes supplementary material
dc.description.abstractBacterial flagellar motility, an important virulence factor, is energized by a rotary motor localized within the flagellar basal body. The rotor module consists of a large framework (the C-ring), composed of the FliG, FliM and FliN proteins. FliN and FliM contacts the FliG torque ring to control the direction of flagellar rotation. We report that structure-based models constrained only by residue coevolution can recover the binding interface of atomic X-ray dimer complexes with remarkable accuracy (approx. 1 angstrom RMSD). We propose a model for FliM-FliN heterodimerization, which agrees accurately with homologous interfaces as well as in situ cross-linking experiments, and hence supports a proposed architecture for the lower portion of the C- ring. Furthermore, this approach allowed the identification of two discrete and interchangeable homodimerization interfaces between FliM middle domains that agree with experimental measurements and might be associated with C-ring directional switching dynamics triggered upon binding of CheY signal protein. Our findings provide structural details of complex formation at the C-ring that have been difficult to obtain with previous methodologies and clarify the architectural principle that underpins the ultra-sensitive allostery exhibited by this ring assembly that controls the clockwise or counterclockwise rotation of flagella.
dc.description.departmentSchool of Natural Sciences and Mathematics
dc.identifier.bibliographicCitationdos Santos, Ricardo Nascimento, Shahid Khan, and Faruck Morcos. 2018. "Characterization of C-ring component assembly in flagellar motors from amino acid coevolution." Royal Society Open Science 5, doi:10.1098/rsos.171854
dc.identifier.issn2054-5703
dc.identifier.urihttps://hdl.handle.net/10735.1/6502
dc.identifier.volume5
dc.language.isoen
dc.publisherRoyal Soc
dc.relation.urihttp://dx.doi.org/10.1098/rsos.171854
dc.rightsCC BY 4.0 (Attribution)
dc.rights©2018 The Authors
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.source.journalRoyal Society Open Science
dc.subjectCoevolution
dc.subjectProteins
dc.subjectFlagella (Microbiology)
dc.subjectVirulence (Microbiology)
dc.titleCharacterization of C-Ring Component Assembly in Flagellar Motors from Amino Acid Coevolution
dc.type.genrearticle

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