Novel Method for the Formation of Monodisperse Superheated Perfluorocarbon Nanodroplets as Activatable Ultrasound Contrast Agents

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dc.contributor.authorDe, Gracia Luxen_US
dc.contributor.authorVezeridis, A. M.en_US
dc.contributor.authorLux, J.en_US
dc.contributor.authorArmstrong, A. M.en_US
dc.contributor.authorSirsi, Shashank R.en_US
dc.contributor.authorHoyt, Kennethen_US
dc.contributor.authorMattrey, R. F.en_US
dc.contributor.utdAuthorSirsi, Shashank R.en_US
dc.contributor.utdAuthorHoyt, Kennethen_US
dc.date.accessioned2018-08-20T16:33:43Z
dc.date.available2018-08-20T16:33:43Z
dc.date.created2017-10-16en_US
dc.date.issued2018-08-20
dc.descriptionIncludes supplementary materialen_US
dc.description.abstractMicrobubble (MB) contrast agents have positively impacted the clinical ultrasound (US) community worldwide. Their use in molecular US imaging applications has been hindered by their limited distribution to the vascular space. Acoustic droplet vaporization (ADV) of nanoscale superheated perfluorocarbon nanodroplets (NDs) demonstrates potential as an extravascular contrast agent that could facilitate US-based molecular theranostic applications. However these agents are metastable and difficult to manufacture with high yields. Here, we report a new formulation technique that yields reliable, narrowly dispersed sub-300 nm decafluorobutane (DFB) or octafluoropropane (OFP)-filled phospholipid-coated NDs that are stable at body temperature, using small volume microfluidization. Final droplet concentration was high for DFB and lower for OFP (>10¹² vs. >10¹⁰ NDs per mL). Superheated ND stability was quantified using tunable resistive pulse sensing (TRPS) and dynamic light scattering (DLS). DFB NDs were stable for at least 2 hours at body temperature (37 °C) without spontaneous vaporization. These NDs are activatable in vitro when exposed to diagnostic US pressures delivered by a clinical system to become visible microbubbles. The DFB NDs were sufficiently stable to allow their processing into functionalized NDs with anti-epithelial cell adhesion molecule (EpCAM) antibodies to target EpCAM positive cells.en_US
dc.description.departmentErik Jonsson School of Engineering and Computer Scienceen_US
dc.identifier.bibliographicCitationDe, Gracia Lux, A. M. Vezeridis, J. Lux, A. M. Armstrong, et al. 2017. "Novel method for the formation of monodisperse superheated perfluorocarbon nanodroplets as activatable ultrasound contrast agents." RSC Advances 7(77): 48561-48568.en_US
dc.identifier.issn2046-2069en_US
dc.identifier.issue77en_US
dc.identifier.urihttp://hdl.handle.net/10735.1/6002
dc.identifier.volume7en_US
dc.language.isoenen_US
dc.publisherRoyal Society of Chemistryen_US
dc.relation.urihttp://dx.doi.org/10.1039/c7ra08971fen_US
dc.rightsCC BY-NC 3.0 (Attribution-NonCommercial) Licenseen_US
dc.rights©2017 The Royal Society of Chemistry.en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/en_US
dc.source.journalRSC Advancesen_US
dc.subjectBubblesen_US
dc.subjectCell adhesionen_US
dc.subjectDropsen_US
dc.subjectDynamic Light Scatteringen_US
dc.subjectPhospholipidsen_US
dc.subjectPhysiologyen_US
dc.subjectVaporizationen_US
dc.subjectEpithelial Cell Adhesion Moleculeen_US
dc.subjectMicrofluidicsen_US
dc.subjectUltrasound contrast mediaen_US
dc.subjectUltrasonicsen_US
dc.titleNovel Method for the Formation of Monodisperse Superheated Perfluorocarbon Nanodroplets as Activatable Ultrasound Contrast Agentsen_US
dc.type.genrearticleen_US

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Sirsi, Shashank R.
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