Constricted Microfluidic Devices to Study the Effects of Transient High Shear Exposure on Platelets

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dc.contributor.ORCID0000-0001-6404-318X (Schmidtke, DW)en_US
dc.contributor.authorAlsmadi, Nesreen Z.en_US
dc.contributor.authorShapiro, Sarah J.en_US
dc.contributor.authorLewis, Christopher S.en_US
dc.contributor.authorSheth, Vinit M.en_US
dc.contributor.authorSnyder, Trevor A.
dc.contributor.authorSchmidtke, David W.
dc.contributor.utdAuthorSnyder, Trevor A.en_US
dc.contributor.utdAuthorSchmidtke, David W.en_US
dc.date.accessioned2018-10-01T20:17:50Z
dc.date.available2018-10-01T20:17:50Z
dc.date.created2017-11-28en_US
dc.date.issued2018-10-01
dc.descriptionFull text access from Treasures at UT Dallas is restricted at this time to current UTD affiliates. The publisher's embargo will be lifted in 2018-12.
dc.description.abstractDue to the critical roles that platelets play in thrombosis during many biological and pathological events, altered platelet function may be a key contributor to altered hemostasis, leading to both thrombotic and hemorrhagic complications. Platelet adhesion at arterial shear rates occurs through binding to von Willebrand Factor via the glycoprotein (GP) GPIb receptor. GPIb binding can induce platelet activation distinguishable by P-selectin (CD62P) surface expression and α(IIb)β₃ activation, resulting in platelet aggregation and formation of the primary hemostatic plug to stop bleeding. Previous studies have used cone and plate viscometers to examine pathologic blood flow conditions, applied shear rates that are relatively low, and examined exposure times that are orders of magnitude longer compared to conditions present in ventricular assist devices, mechanical heart valves, or pathologic states such as stenotic arteries. Here, we evaluate the effect of short exposure to high shear on granule release and receptor shedding utilizing a constricted microfluidic device in conjunction with flow cytometry and enzyme-linked immunosorbent assay. In this study, platelets were first perfused through microfluidic channels capable of producing shear rates of 80 000-100 000 s⁻¹ for exposure times of 0-73 ms. We investigated platelet activation by measuring the expression level of CD62P (soluble and surface expressed), platelet factor 4 (PF4), and beta-thromboglobulin (βTG). In addition, we measured potential platelet receptor shedding of GPVI and GPIb using flow cytometry. The results showed that a single pass to high shear with short exposure times (milliseconds) had no effect on the levels of CD62P, GPVI and GPIb, or on the release of alpha granule content (PF4, βTG, and sP-selectin). Published by AIP Publishing.en_US
dc.description.departmentErik Jonsson School of Engineering and Computer Scienceen_US
dc.description.sponsorship"Research reported in this publication was supported by the National Heart, Lung, and Blood Institute of the National Institutes of Health under Award Number R21HL115601 and R21HL132286."en_US
dc.identifier.bibliographicCitationAlsmadi, Nesreen Z., Sarah J. Shapiro, Christopher S. Lewis, Vinit M. Sheth, et al. 2017. "Constricted microfluidic devices to study the effects of transient high shear exposure on platelets." Biomicrofluidics 11(6), doi:10.1063/1.4989386en_US
dc.identifier.issn1932-1058en_US
dc.identifier.issue6en_US
dc.identifier.urihttp://hdl.handle.net/10735.1/6179
dc.identifier.volume11en_US
dc.language.isoenen_US
dc.publisherAmer Inst Physicsen_US
dc.relation.urihttp://dx.doi.org/10.1063/1.4989386en_US
dc.rights©2017 American Institute of Physicsen_US
dc.source.journalBiomicrofluidicsen_US
dc.subjectVon Willebrand factoren_US
dc.subjectHeart-Assist Devicesen_US
dc.subjectGlycoproteinsen_US
dc.subjectHeart-valvesen_US
dc.subjectIn Vitro Techniquesen_US
dc.subjectStress (Physiology)en_US
dc.subjectBlood platelets--Aggregationen_US
dc.titleConstricted Microfluidic Devices to Study the Effects of Transient High Shear Exposure on Plateletsen_US
dc.type.genrearticleen_US

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