MicroRNA Expression Profiling of Pancreatic Cancer Cell Line L3.6p1 Following B7-H4 Knockdown

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dc.contributor.authorQian, Yunen_US
dc.contributor.authorFeng, Liminen_US
dc.contributor.authorWu, Weigenen_US
dc.contributor.authorWeng, Tianhaoen_US
dc.contributor.authorHu, Chenyuen_US
dc.contributor.authorHong, Boen_US
dc.contributor.authorWang, Frederick X. C.en_US
dc.contributor.authorShen, Lingweien_US
dc.contributor.authorWang, Qien_US
dc.contributor.authorJin, Xinen_US
dc.contributor.authorYao, Hangpingen_US
dc.contributor.utdAuthorWang, Frederick X. C.en_US
dc.date.accessioned2018-10-22T19:26:14Z
dc.date.available2018-10-22T19:26:14Z
dc.date.created2018-10-22en_US
dc.date.issued2018-10-22
dc.description.abstractBackground/Aims: Co-stimulating molecule B7-H4 regulates T cell-mediated immune responses, participates in tumor immune escape, and promotes the proliferation and metastasis of pancreatic cancer cells. However, the specific mechanisms are unclear. MicroRNAs (miRNAs) participated in the pathogenesis and progression of cancer. Methods: In this study, a microarray technique was used to screen B7-H4-related differentially expressed miRNAs in a pancreatic cancer cell line find those associated with pancreatic cancer. Using a miRCURY (TM) LNA Array approach, we compared the miRNA expression profiles of L3.6p1 pancreatic cancer cells transfected with B7-H4 siRNA for 72 h with those transfected with non-target siRNAs. Results: B7-H4 siRNA significantly up-regulated 57 miRNAs and down-regulated 14 miRNAs. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway analysis of predicted miRNA targets showed that these genes were mainly involved in protein binding, pathways in cancer, mitogen-activated protein kinase (MAPK) signaling pathway, and phosphatidylinositol 3-kinase-Akt (PI3K-Akt) signaling pathway. Conclusions: This is the first description of target genes of B7-H4, showing that miRNAs participate in the B7-H4 mediated regulation of oncogenicity and pathogenesis of pancreatic cancer. These results may help us better understand the role of B7-H4 in the progression of pancreatic cancer and its possible mechanisms. We also provide novel biomarkers for potential treatments of pancreatic cancer.en_US
dc.description.departmentErik Jonsson School of Engineering and Computer Scienceen_US
dc.description.sponsorshipNational Natural Science Foundation of China [grant number 30972777]; and the Zhejiang Provincial Natural Science Foundation of China [grant number LY15H190002 and LQ16H160005]; and the technological research project for public welfare of Shaoxing [grant number 2014B70051] of China.en_US
dc.identifier.bibliographicCitationQian, Yun, Limin Feng, Weigen Wu, Tianhao Weng, et al. 2017. "MicroRNA expression profiling of pancreatic cancer cell line L3.6p1 following B7-H4 knockdown." Cellular Physiology and Biochemistry 44(2), 494-504, doi:10.1159/000485086en_US
dc.identifier.issn1015-8987en_US
dc.identifier.issue2en_US
dc.identifier.urihttp://hdl.handle.net/10735.1/6205
dc.identifier.volume44en_US
dc.language.isoenen_US
dc.publisherKarger AGen_US
dc.relation.urihttp://dx.doi.org/10.1159/000485086
dc.rightsCC BY-NC-ND 4.0 (Attribution-NonCommercial-NoDerivatives)en_US
dc.rights©2017 The Authorsen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.source.journalCellular Physiology and Biochemistryen_US
dc.subjectAdenocarcinomaen_US
dc.subjectCancer cellsen_US
dc.subjectBreast--Canceren_US
dc.subjectStem cellsen_US
dc.subjectApoptosisen_US
dc.subjectMetastasisen_US
dc.subjectCytologyen_US
dc.subjectPhysiologyen_US
dc.subjectMicroRNAen_US
dc.subjectPancreatic Neoplasmsen_US
dc.subjectCancer invasivenessen_US
dc.titleMicroRNA Expression Profiling of Pancreatic Cancer Cell Line L3.6p1 Following B7-H4 Knockdownen_US
dc.type.genrearticleen_US

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