Preparation of Immunochromatographic Strips for Rapid Detection of Early Secreted Protein ESAT-6 and Culture Filtrate Protein CFP-10 from Mycobacterium Tuberculosis

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dc.contributor.authorWu, Xiaoxinen_US
dc.contributor.authorWang, Yepingen_US
dc.contributor.authorWeng, Tianhaoen_US
dc.contributor.authorHu, Chenyuen_US
dc.contributor.authorWang, Frederick X. C.en_US
dc.contributor.authorWu, Zhigangen_US
dc.contributor.authorYu, Dongshanen_US
dc.contributor.authorLu, Huoquanen_US
dc.contributor.authorYao, Hangpingen_US
dc.contributor.utdAuthorWang, Frederick X. C.en_US
dc.date.accessioned2018-11-05T21:00:54Z
dc.date.available2018-11-05T21:00:54Z
dc.date.created2017-12en_US
dc.date.issued2018-11-05
dc.descriptionIncludes supplementary materialen_US
dc.description.abstractThe early secreted protein early secretory antigenic target 6(ESAT-6) and the culture filtrate protein 10 (CFP-10) are 2 antigens that are specific to Mycobacterium tuberculosis. These 2 antigens are good targets for tuberculosis (TB) detection.To rapidly diagnose TB across a variety of samples, we developed colloidal gold immunochromatographic strips (ICSs) based on ESAT-6 and CFP-10.The strips were evaluated using 233 samples, including sputum, plasma, and pleural effusion samples.The positive detection rates for ICSs for ESAT-6 and CFP-10 in sputum (culture-positive for M tuberculosis) were 100% and 91.2%, respectively. The positive detection rates for ICSs for ESAT-6 and CFP-10 in plasma were 34.1% and 29.4%, respectively. The positive detection rates for ICSs for ESAT-6 and CFP-10 in pleural effusion were 64.7% and 55.9%, respectively. Experimental analysis of culture supernatant showing that the ICS developed for ESAT-6 had a sensitivity of 100% and a specificity of 91.2%. While the ICS developed for CFP-10 had a sensitivity of 91.2% and a specificity of 88.2%.The validity of the test is limited by source of sample. The technique is sensitive and specific for samples in sputum and culture media but not for plasma or pleural effusion samples. Detection of M tuberculosis using ICSs is rapid, simple, and relatively effective; thus, ICSs are a potential screening tool for TB.en_US
dc.description.departmentErik Jonsson School of Engineering and Computer Scienceen_US
dc.identifier.bibliographicCitationWu, Xiaoxin, Yeping Wang, Tianhao Weng, Chenyu Hu, et al. 2017. "Preparation of immunochromatographic strips for rapid detection of early secreted protein ESAT-6 and culture filtrate protein CFP-10 from Mycobacterium tuberculosis." Medicine 96(51), doi:10.1097/MD.0000000000009350en_US
dc.identifier.issn0025-7974en_US
dc.identifier.issue51en_US
dc.identifier.urihttp://hdl.handle.net/10735.1/6257
dc.identifier.volume96en_US
dc.language.isoenen_US
dc.publisherLippincott Williams & Wilkinsen_US
dc.relation.urihttp://dx.doi.org/10.1097/MD.0000000000009350
dc.rightsCC BY 4.0 (Attribution)en_US
dc.rights©2017 The Authorsen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.source.journalMedicineen_US
dc.subjectSmear microscopyen_US
dc.subjectDiagnosisen_US
dc.subjectBiological Assayen_US
dc.subjectAntigensen_US
dc.subjectInfectionen_US
dc.subjectImmunochromatographyen_US
dc.subjectESAT-6 proteinen_US
dc.subjectMycobacterium tuberculosisen_US
dc.subjectCFP-10 protein, Mycobacterium tuberculosisen_US
dc.subjectTuberculosis, Pulmonaryen_US
dc.subjectPleural Effusionen_US
dc.titlePreparation of Immunochromatographic Strips for Rapid Detection of Early Secreted Protein ESAT-6 and Culture Filtrate Protein CFP-10 from Mycobacterium Tuberculosisen_US
dc.type.genrearticleen_US

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