Simultaneous Lancet-Free Monitoring of Alcohol and Glucose from Low-Volumes of Perspired Human Sweat

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dc.contributor.ISNI0000 0001 2765 4678 (Prasad, S)
dc.contributor.ORCID0000-0002-2404-3801 (Prasad, S)
dc.contributor.authorBhide, Ashlesha
dc.contributor.authorMuthukumar, Sriram
dc.contributor.authorSaini, Amreek
dc.contributor.authorPrasad, Shalini
dc.contributor.utdAuthorBhide, Ashlesha
dc.contributor.utdAuthorSaini, Amreek
dc.contributor.utdAuthorPrasad, Shalini
dc.date.accessioned2019-06-28T15:52:11Z
dc.date.available2019-06-28T15:52:11Z
dc.date.created2018-04-25
dc.description.abstractA lancet-free, label-free biosensor for simultaneous detection of sweat glucose and alcohol was demonstrated using zinc oxide thin films integrated into a nanoporous flexible electrode system. Sensing was achieved from perspired human sweat at low volumes (1-3μL), comparable to ambient conditions without external stimulation. Zinc oxide thin film electrodes were surface functionalized with alcohol oxidase enzyme and with glucose oxidase enzyme towards developing an affinity biosensor specific to the physiological relevant range of alcohol comprising of 0-2 drinks (0-50mg/dl) and physiologically relevant range of glucose ranging from hypo- to hyper-glycaemia (50- 130mg/dl) in perspired human sweat. Sensing was achieved by measuring impedance changes associated with alcohol and glucose binding onto the sensor interface using electrochemical impedance spectroscopy with a dynamic range from 0.01-200mg/dl and a limit of detection of 0.01mg/dl for alcohol in human sweat. Sensor calibration in synthetic sweat containing interferents (25-200mg/dl) and comparison using regression and Bland-Altman analysis of sweat sensor performance was done with BACtrack. Combinatorial detection of glucose and ethanol in perspired human sweat and comparison of sweat sensor performance with Accu-Chek blood glucose monitoring system that we expect would be relevant for pre-diabetics and diabetics for monitoring their glucose levels and alcohol consumption.
dc.description.departmentErik Jonsson School of Engineering and Computer Science
dc.description.sponsorshipNational Institute on Alcohol Abuse and Alcoholism of the National Institutes of Health (R43AA026114).
dc.identifier.bibliographicCitationBhide, Ashlesha, Sriram Muthukumar, Amreek Saini, and Shalini Prasad. 2018. "Simultaneous lancet-free monitoring of alcohol and glucose from low-volumes of perspired human sweat." Scientific Reports 8, doi:10.1038/s41598-018-24543-4
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/10735.1/6619
dc.identifier.volume8
dc.relation.urihttps://doi.org/10.1038/s41598-018-24543-4
dc.rightsCC BY 4.0 (Attribution)
dc.rights©2018 The Authors
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.source.journalScientific Reports
dc.subjectBiochemical markers
dc.subjectPerspiration
dc.subjectGlucose
dc.subjectAlcohol
dc.subjectZinc oxide
dc.subjectBiosensors
dc.titleSimultaneous Lancet-Free Monitoring of Alcohol and Glucose from Low-Volumes of Perspired Human Sweat
dc.type.genrearticle

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