Fluorescent stereo microscopy for 3D surface profilometry and deformation mapping

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dc.contributor.authorHu, Zhenxingen_US
dc.contributor.authorLuo, Huiyangen_US
dc.contributor.authorDu, Yingjieen_US
dc.contributor.authorLu, Hongbingen_US
dc.date.accessioned2014-07-18T19:15:20Z
dc.date.available2014-07-18T19:15:20Z
dc.date.created2013-05-07
dc.date.submitted2013-03-22en_US
dc.description.abstractRecently, mechanobiology has received increased attention. For investigation of biofilm and cellular tissue, measurements of the surface topography and deformation in real-time are a pre-requisite for understanding the growth mechanisms. In this paper, a novel three-dimensional (3D) fluorescent microscopic method for surface profilometry and deformation measurements is developed. In this technique a pair of cameras are connected to a binocular fluorescent microscope to acquire micrographs from two different viewing angles of a sample surface doped or sprayed with fluorescent microparticles. Digital image correlation technique is used to search for matching points in the pairing fluorescence micrographs. After calibration of the system, the 3D surface topography is reconstructed from the pair of planar images. When the deformed surface topography is compared with undeformed topography using fluorescent microparticles for movement tracking of individual material points, the full field deformation of the surface is determined. The technique is demonstrated on topography measurement of a biofilm, and also on surface deformation measurement of the biofilm during growth. The use of 3D imaging of the fluorescent microparticles eliminates the formation of bright parts in an image caused by specular reflections. The technique is appropriate for non-contact, full-field and real-time 3D surface profilometry and deformation measurements of materials and structures at the microscale.;en_US
dc.description.sponsorship"We acknowledge the support of DOE NEUP 09-818. We also thank the additional support from NSF DMR-0907291, CMMI-1031829, CMMI-1132174, and ECCS-1307997, and NIH R01 EB013212 and R01 DC011585. We appreciate Dr. Munehiro Asally and Dr. Gürol M. Süel at the University of California, San Diego for providing the biofilm samples."en_US
dc.identifier.citationHu, Zhenxing, Huiyang Luo, Yingjie Du, and Hongbing Lu. 2013. "Fluorescent stereo microscopy for 3D surface profilometry and deformation mapping." Optics Express 21(10): 11808-11818en_US
dc.identifier.issn1094-4087en_US
dc.identifier.issue10en_US
dc.identifier.startpage11808en_US
dc.identifier.urihttp://hdl.handle.net/10735.1/3732
dc.identifier.volume21en_US
dc.relation.urihttp://dx.doi.org/10.1364/OE.21.011808en_US
dc.rights©2013 Optical Society of Americaen_US
dc.source.journalOptics Expressen_US
dc.subjectSurface topographyen_US
dc.subjectThree dimensional sensingen_US
dc.subjectFluorescence microscopyen_US
dc.subjectThree dimensional microscopyen_US
dc.subjectBiomedical materialsen_US
dc.titleFluorescent stereo microscopy for 3D surface profilometry and deformation mappingen_US
dc.typetexten_US
dc.type.genrearticleen_US

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