Histone Chaperone Nap1 Is a Major Regulator of Histone H2A-H2B Dynamics at the Inducible Gal Locus

dc.contributor.authorChen, Xuen_US
dc.contributor.authorD'Arcy, Sheenaen_US
dc.contributor.authorRadebaugh, Catherine A.en_US
dc.contributor.authorKrzizike, Daniel D.en_US
dc.contributor.authorGiebler, Holli A.en_US
dc.contributor.authorHuang, Liangquanen_US
dc.contributor.authorNyborg, Jennifer K.en_US
dc.contributor.authorLuger, Karolinen_US
dc.contributor.authorStargell, Laurie A.en_US
dc.contributor.utdAuthorD'Arcy, Sheenaen_US
dc.date.accessioned2017-11-29T22:10:38Z
dc.date.available2017-11-29T22:10:38Z
dc.date.created2016-02-16en_US
dc.date.issued2016-02-16en_US
dc.description.abstractHistone chaperones, like nucleosome assembly protein 1 (Nap1), play a critical role in the maintenance of chromatin architecture. Here, we use the GAL locus in Saccharomyces cerevisiae to investigate the influence of Nap1 on chromatin structure and histone dynamics during distinct transcriptional states. When the GAL locus is not expressed, cells lacking Nap1 show an accumulation of histone H2A-H2B but not histone H3-H4 at this locus. Excess H2A-H2B interacts with the linker DNA between nucleosomes, and the interaction is independent of the inherent DNA-binding affinity of H2A-H2B for these particular sequences as measured in vitro. When the GAL locus is transcribed, excess H2A-H2B is reversed, and levels of all chromatinbound histones are depleted in cells lacking Nap1. We developed an in vivo system to measure histone exchange at the GAL locus and observed considerable variability in the rate of exchange across the locus in wild-type cells. We recapitulate this variability with in vitro nucleosome reconstitutions, which suggests a contribution of DNA sequence to histone dynamics. We also find that Nap1 is required for transcription-dependent H2A-H2B exchange. Altogether, these results indicate that Nap1 is essential for maintaining proper chromatin composition and modulating the exchange of H2A-H2B in vivo.en_US
dc.description.sponsorshipNational Institute of General Medical Sciences (no. 100000057); Howard Hughes Medical Institute (no. 100000011); National Science Foundation Division of Molecular and Cellular Biosciences (no. 100000076)en_US
dc.identifier.bibliographicCitationChen, Xu, Sheena D'Arcy, Catherine A. Radebaugh, Daniel D. Krzizike, et al. 2016. "Histone Chaperone Nap1 Is a Major Regulator of Histone H2A-H2B Dynamics at the Inducible GAL Locus." Molecular and Cellular Biology 36(8), doi:10.1128/MCB.00835-15en_US
dc.identifier.issn0270-7306en_US
dc.identifier.issue8en_US
dc.identifier.urihttp://hdl.handle.net/10735.1/5597
dc.identifier.volume36en_US
dc.language.isoenen_US
dc.publisherAmer Soc Microbiologyen_US
dc.relation.urihttp://dx.doi.org/10.1128/MCB.00835-15
dc.rights©2016 American Society for Microbiology. All Rights Reserved.en_US
dc.sourceMolecular and Cellular Biology
dc.subjectNucleosomesen_US
dc.subjectRNA Polymerase IIen_US
dc.subjectSaccharomyces cerevisiaeen_US
dc.subjectChromatinen_US
dc.subjectSaccharomycetalesen_US
dc.subjectTATA Boxen_US
dc.subjectTranscriptional Activationen_US
dc.subjectHistonesen_US
dc.titleHistone Chaperone Nap1 Is a Major Regulator of Histone H2A-H2B Dynamics at the Inducible Gal Locusen_US
dc.type.genrearticleen_US

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