Integrated Omics Study Delineates the Dynamics of Lipid Droplets in Rhodococcus Opacus PD630

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dc.contributor.ISNI0000 0001 1707 1372 (Zhang, MQ)
dc.contributor.LCNA99086074‏ ‎(Zhang, MQ)
dc.contributor.authorChen, Yongen_US
dc.contributor.authorDing, Yunfengen_US
dc.contributor.authorYang, Lien_US
dc.contributor.authorYu, Jinhaien_US
dc.contributor.authorLiu, Guimingen_US
dc.contributor.authorWang, Xuminen_US
dc.contributor.authorZhang, Shuyanen_US
dc.contributor.authorZhang, Michael Q.en_US
dc.contributor.authorLi, Yandaen_US
dc.contributor.utdAuthorZhang, Michael Q.en_US
dc.date.accessioned2014-07-14T19:36:40Z
dc.date.available2014-07-14T19:36:40Z
dc.date.created2013-10-22en_US
dc.date.issued2013-10-22en_US
dc.date.submitted2013-08-06en_US
dc.descriptionIncludes supplementary material.en_US
dc.description.abstractRhodococcus opacus strain PD630 (R. opacus PD630), is an oleaginous bacterium, and also is one of few prokaryotic organisms that contain lipid droplets (LDs). LD is an important organelle for lipid storage but also intercellular communication regarding energy metabolism, and yet is a poorly understood cellular organelle. To understand the dynamics of LD using a simple model organism, we conducted a series of comprehensive omics studies of R. opacus PD630 including complete genome, transcriptome and proteome analysis. The genome of R. opacus PD630 encodes 8947 genes that are significantly enriched in the lipid transport, synthesis and metabolic, indicating a super ability of carbon source biosynthesis and catabolism. The comparative transcriptome analysis from three culture conditions revealed the landscape of gene-altered expressions responsible for lipid accumulation. The LD proteomes further identified the proteins that mediate lipid synthesis, storage and other biological functions. Integrating these three omics uncovered 177 proteins that may be involved in lipid metabolism and LD dynamics. A LD structure-like protein LPD06283 was further verified to affect the LD morphology. Our omics studies provide not only a first integrated omics study of prokaryotic LD organelle, but also a systematic platform for facilitating further prokaryotic LD research and biofuel development.en_US
dc.identifier.bibliographicCitationChen, Yong, Yunfeng Ding, Li Yang, Jinhai Yu, et al. 2014. "Integrated omics study delineates the dynamics of lipid droplets in Rhodococcus opacus PD630." Nucleic Acids Research 42(2): 1052-1064.en_US
dc.identifier.issn0305-1048en_US
dc.identifier.issue2en_US
dc.identifier.startpage1052en_US
dc.identifier.urihttp://hdl.handle.net/10735.1/3684
dc.identifier.volume42en_US
dc.language.isoenen_US
dc.publisherOxford University Pressen_US
dc.relation.urihttp://dx.doi.org/10.1093/nar/gkt932
dc.rightsCC BY 3.0 (Attribution)en_US
dc.rights©2013 The Authors.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/
dc.sourceNucleic Acids Research
dc.subject5-CMLD protein, Rhodococcus opacus PD630en_US
dc.subjectChlamydomonas reinhardtiien_US
dc.subjectEndoplasmic reticulumen_US
dc.subjectMetabolic syndromeen_US
dc.subjectProteinsen_US
dc.subjectGenomesen_US
dc.subjectGenesen_US
dc.titleIntegrated Omics Study Delineates the Dynamics of Lipid Droplets in Rhodococcus Opacus PD630en_US
dc.typeTexten_US
dc.type.genreArticleen_US

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Zhang, Michael Q.
CSB Research