Pyruvate Dehydrogenase Kinase Is a Metabolic Checkpoint for Polarization of Macrophages to the M1 Phenotype

dc.contributor.ORCID0000-0002-7829-1480 (Kim, J-w)
dc.contributor.authorMin, B. -K
dc.contributor.authorPark, S.
dc.contributor.authorKang, H. -J
dc.contributor.authorKim, D. W.
dc.contributor.authorHam, H. J.
dc.contributor.authorHa, C. -M
dc.contributor.authorChoi, B. -J
dc.contributor.authorKim, Jung-whan
dc.contributor.utdAuthorKim, Jung-whan
dc.date.accessioned2020-06-17T14:23:53Z
dc.date.available2020-06-17T14:23:53Z
dc.date.issued2019-05-07
dc.descriptionIncludes supplementary material
dc.description.abstractMetabolic reprogramming during macrophage polarization supports the effector functions of these cells in health and disease. Here, we demonstrate that pyruvate dehydrogenase kinase (PDK), which inhibits the pyruvate dehydrogenase-mediated conversion of cytosolic pyruvate to mitochondrial acetyl-CoA, functions as a metabolic checkpoint in M1 macrophages. Polarization was not prevented by PDK2 or PDK4 deletion but was fully prevented by the combined deletion of PDK2 and PDK4; this lack of polarization was correlated with improved mitochondrial respiration and rewiring of metabolic breaks that are characterized by increased glycolytic intermediates and reduced metabolites in the TCA cycle. Genetic deletion or pharmacological inhibition of PDK2/4 prevents polarization of macrophages to the M1 phenotype in response to inflammatory stimuli (lipopolysaccharide plus IFN-γ). Transplantation of PDK2/4-deficient bone marrow into irradiated wild-type mice to produce mice with PDK2/4-deficient myeloid cells prevented M1 polarization, reduced obesity-associated insulin resistance, and ameliorated adipose tissue inflammation. A novel, pharmacological PDK inhibitor, KPLH1130, improved high-fat diet-induced insulin resistance; this was correlated with a reduction in the levels of pro-inflammatory markers and improved mitochondrial function. These studies identify PDK2/4 as a metabolic checkpoint for M1 phenotype polarization of macrophages, which could potentially be exploited as a novel therapeutic target for obesity-associated metabolic disorders and other inflammatory conditions.
dc.description.departmentSchool of Natural Sciences and Mathematics
dc.description.sponsorship"This work was supported by a grant of Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health and Welfare, the Republic of Korea (HI16C1501) & Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2017R1A2B3006406), and Bio & Medical Technology Development Program of the National Research Foundation (NRF) & funded by the Korean government (MSIP&MOHW) (No. NRF-2016M3A9B6902872).
dc.identifier.bibliographicCitationMin, B. -K, S. Park, H. -J Kang, D. W. Kim, et al. 2019. "Pyruvate Dehydrogenase Kinase Is a Metabolic Checkpoint for Polarization of Macrophages to the M1 Phenotype." Frontiers in Immunology 10: art. 944, doi: 10.3389/fimmu.2019.00944
dc.identifier.issn1664-3224
dc.identifier.urihttp://dx.doi.org/10.3389/fimmu.2019.00944
dc.identifier.urihttps://hdl.handle.net/10735.1/8671
dc.identifier.volume10
dc.language.isoen
dc.publisherFrontiers Media S.A.
dc.rightsCC BY 4.0 (Attribution)
dc.rights©2019 The Authors
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.source.journalFrontiers in Immunology
dc.subjectDichloroacetate
dc.subjectInflammation
dc.subjectInsulin resistance
dc.subject.meshDiet, High-Fat
dc.subject.meshMacrophages
dc.subject.meshPyruvate Dehydrogenase (Acetyl-Transferring) Kinase
dc.titlePyruvate Dehydrogenase Kinase Is a Metabolic Checkpoint for Polarization of Macrophages to the M1 Phenotype
dc.type.genrearticle
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