Regulation of Acetate Utilization by Monocarboxylate Transporter 1 (MCT1) in Hepatocellular Carcinoma (HCC)




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Cognizant Communication Corporation


Altered energy metabolism is a biochemical fingerprint of cancer cells. Hepatocellular carcinoma (HCC) shows reciprocal [¹⁸F]fluorodeoxyglucose (FDG) and [¹¹C]acetate uptake, as revealed by positron emission tomography/computed tomography (PET/CT). Previous studies have focused on the role of FDG uptake in cancer cells. In this study, we evaluated the mechanism and roles of [¹¹C]acetate uptake in human HCCs and cell lines. The expression of monocarboxylate transporters (MCTs) was assessed to determine the transporters of [¹¹C]acetate uptake in HCC cell lines and human HCCs with different [¹¹C]acetate uptake. Using two representative cell lines with widely different [¹¹C]acetate uptake (HepG2 for high uptake and Hep3B for low uptake), changes in [¹¹C]acetate uptake were measured after treatment with an MCT1 inhibitor or MCT1- targeted siRNA. To verify the roles of MCT1 in cells, oxygen consumption rate and the amount of lipid synthesis were measured. HepG2 cells with high [¹¹C]acetate uptake showed higher MCT1 expression than other HCC cell lines with low [¹¹C]acetate uptake. MCT1 expression was elevated in human HCCs with high [¹¹C] acetate uptake compared to those with low [¹¹C]acetate uptake. After blocking MCT1 with AR-C155858 or MCT1 knockdown, [¹¹C]acetate uptake in HepG2 cells was significantly reduced. Additionally, inhibition of MCT1 suppressed mitochondrial oxidative phosphorylation, lipid synthesis, and cellular proliferation in HCC cells with high [¹¹C]acetate uptake. MCT1 may be a new therapeutic target for acetate-dependent HCCs with high [¹¹C]acetate uptake, which can be selected by [¹¹C]acetate PET/CT imaging in clinical practice.



Carcinoma, Hepatocellular, Monocarboxylic Acid Transporters, Positron-Emission Tomography, Acetic acid, Small interfering RNA, Cell proliferation, Gene Knockdown Techniques, Gene targeting, Humans, Lipogenesis, Oxygen consumption (Physiology), Phosphorylation, Liver--Cancer, Tomography, Emission, Oxygen Consumption

"This work was supported by the National Research Foundation of Korea grants funded by the Korean government (MSIP) (NRF-2011-0030086 and NRF- 2016R1E1A1A01943303), and the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Science, ICT, and Future Planning (2012R1A1A3008042)."


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