Atmaramani, RahulBlack, Brian J.Lam, Kevin H.Sheth, Vinit M.Pancrazio, Joseph J.Schmidtke, David W.Alsmadi, Nesreen Zoghoul2019-10-312019-10-312019-02-212072-666Xhttps://hdl.handle.net/10735.1/7053In vitro systems comprised of wells interconnected by microchannels have emerged as a platform for the study of cell migration or multicellular models. In the present study, we systematically evaluated the effect of microchannel width on spontaneous myoblast migration across these microchannels-from the proximal to the distal chamber. Myoblast migration was examined in microfluidic devices with varying microchannel widths of 1.5-20 µm, and in chips with uniform microchannel widths over time spans that are relevant for myoblast-to-myofiber differentiation in vitro. We found that the likelihood of spontaneous myoblast migration was microchannel width dependent and that a width of 3 µm was necessary to limit spontaneous migration below 5% of cells in the seeded well after 48 h. These results inform the future design of Polydimethylsiloxane (PDMS) microchannel-based co-culture platforms as well as future in vitro studies of myoblast migration. © 2019 by the authors.enCC BY 4.0 (Attribution)©2019 The Authorshttp://creativecommons.org/licenses/by/4.0/MicrofabricationMicrofluidicsMyoblastsPolydimethylsiloxanePulse-duration modulationSiliconeCell migrationarticleAtmaramani, R., B. J. Black, K. H. Lam, V. M. Sheth, et al. 2019. "The effect of microfluidic geometry on myoblast migration." Micromachines 10(2): art. 143, doi: 10.3390/mi10020143102