Bharadwaj, AnushaKesinger, Matthew R.Shoura, Massa J.Vetcher, AlexandreLevene, Stephen D.2012-06-192012-06-192012-05-152012-05-15http://hdl.handle.net/10735.1/2140New techniques are needed in order to study the effects of DNA supercoiling on loop-mediated regulation. The thermodynamics of DNA looping depend periodically on the helical phasing between recognition sites. A long-term goal is to provide enabling technologies for ensemble and single-molecule FRET studies of DNA looping mediated by lac repressor in vitro and in vivo. Other applications of the methodology include engineering of DNA substrates for studies of site-specific recombination and other enzyme systemsCC BY-NC 3.0 (Attribution-NonCommercial)©2012 The Authorshttp://creativecommons.org/licenses/by-nc/4.0/http://creativecommons.org/licenses/by-nc/3.0/DNAFluorescence resonance energy transferRecombination, geneticDNA-protein interactionsTextShoura, M. J., A. A. Vetcher, S. M. Giovan, F. Bardai, A. Bharadwaj, M. R. Kesinger, and S. D. Levene. 2012. "Measurements of DNA-Loop Formation Via Cre-Mediated Recombination." Nucleic Acids Research 40 (15): 7452-7464.