Wheelis, Sutton E.Montaño-Figueroa, Ana GabrielaQuevedo-Lopez, Manuel A.Rodrigues, Danieli C.2019-07-122019-07-122018-08-151523-0899https://hdl.handle.net/10735.1/6697Full text access from Treasures at UT Dallas is restricted to current UTD affiliates (use the provided Link to Article). Non UTD affiliates will find the web address for this item by clicking the "Show full item record" link, copying the "dc.relation.uri" metadata and pasting it into a browser.Background: Previous studies have concluded that certain titanium oxide (TiO₂) surface properties promote bone-forming cell attachment. However, no comprehensive studies have investigated the effects of TiO₂ surface and film morphology on hard and soft tissues. Purpose: The aim of this study is to understand the effects of TiO₂ morphology on the proliferation and differentiation of murine preosteoblasts (MC3T3-E1) and proliferation of human gingival fibroblasts (HGF-1) using in vitro experiments. Materials and Methods: Samples were fabricated with several TiO₂ thickness and crystalline structure to mimic various dental implant surfaces. in vitro analysis was performed for 1, 3, and 7 days on these samples to assess the viability of MC3T3-E1 and HGF-1 cells in contact with the modified oxide surfaces. Results: Results showed that HGF-1 cells exhibited no significant difference in viability on modified oxide surfaces versus a titanium control across experiments. MC3T3-E1 cells exhibited a significantly higher viability for the modified oxide surface in 1 day experiments, but not in 3 or 7 day experiments. Alkaline phosphatase expression in MC3T3-E1 was not significantly different on modified oxide surfaces versus the control across all experiments. A slight positive trend in viability was observed for cells in contact with rougher modified oxide surfaces versus a titanium control in both cell types. Conclusions: These observations suggest that crystallinity and thickness do not affect the long-term viability of hard or soft tissue cells when compared to a cpTi surface. Therefore, treatments like anodization on implant components may not directly affect the attachment of hard or soft tissue cells in vivo. © 2018 Wiley Periodicals, Inc.en©2018 Wiley Periodicals, Inc.Cell SurvivalTitanium dioxideOsteoblastsFibroblasts--GingivalarticleWheelis, S. E., A. G. Montaño-Figueroa, M. Quevedo-Lopez, and D. C. Rodrigues. 2018. "Effects of titanium oxide surface properties on bone-forming and soft tissue-forming cells." Clinical Implant Dentistry and Related Research 20(5): 838-847, doi:10.1111/cid.12656205