Pantano, Paul
Permanent URI for this collectionhttps://hdl.handle.net/10735.1/3995
Paul Pantano is an Associate Professor of Chemistry. He is also an affiliated faculty member of the Alan G. MacDiarmid NanoTech Institute. His interest is the "development of elegant analytical techniques and methodologies to understand complex chemical systems." A focus of his work is the "intracellular response and fate of carbon nanomaterials (e.g., carbon nanotubes and graphene oxide), and advancing the diagnostic and therapeutic applications of carbon nanomaterials." Learn more about Dr. Pantano here.
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Item Acute and Chronic Toxicity to Daphnia Magna of Colloidal Silica Nanoparticles in a Chemical Mechanical Planarization Slurry after Polishing a Gallium Arsenide Wafer(Elsevier Science BV, 2018-12-29) Karimi, Sarah; Troeung, Meiline; Wang, Ruhung; Draper, Rockford K.; Pantano, Paul; Crawford, Steven; Aravamudhan, Shyam; 0000-0001-5535-8358 (Pantano, P); Karimi, Sarah; Troeung, Meiline; Wang, Ruhung; Draper, Rockford K.; Pantano, PaulSemiconductor chip manufacturers use slurries of metal oxide nanoparticles (NPs) as abrasives in chemical mechanical planarization (CMP) processes on wafers containing films of III/V semiconducting materials. Assessing the toxicity of these specialized NPs is challenging not only because commercial slurries may contain undefined toxic constituents, but because CMP processes can change the physical and chemical properties of the NPs. Herein, the fresh water flea Daphnia magna (D. magna) was used to assess the effects of Ultra-Sol (R) 200S CMP slurry containing similar to 30-nm colloidal silica (c-SiO₂) NPs before (pristine) and after (spent) a GaAs wafer was polished with an extreme arm-pressure of 5 psi. In the acute 96-hour toxicity assessments, both the pristine and spent slurries at 4.0 mg/mL c-SiO₂ NPs had little effect on D. magna morbidity and body sizes. In the chronic 21-day toxicity assessments, neither slurry at 0.10 mg/mL c-SiO₂ NPs was toxic, but both slurries lead to a modest (9-10%) increase in D. magna body sizes and a significant (similar to 2-fold) increase in reproductive output, indicative of a positive hormetic response. Identical increases in D. magna body sizes and reproductive output were observed with a supernatant of the pristine slurry, in the absence of the c-SiO₂ NPs, indicating that soluble material in the pristine supernatant contributed to the hormetic response, which suggests that the soluble material may also contribute to the hormetic response of the spent material.Item Acute and Chronic Toxicity of Metal Oxide Nanoparticles in Chemical Mechanical Planarization Slurries with Daphnia Magna(Royal Society of Chemistry) Karimi, Sarah; Troeung, Meiline; Wang, Ruhung; Draper, Rockford K.; Pantano, Paul; 0000-0001-5535-8358 (Pantano, P); Karimi, Sarah; Troeung, Meiline; Wang, Ruhung; Draper, Rockford K.; Pantano, PaulThe semiconductor manufacturing industry uses metal oxide nanoparticles (NPs), including colloidal silica (c-SiO₂), fumed silica (f-SiO₂), ceria (CeO₂), and alumina (Al₂O₃), as abrasives in chemical mechanical planarization (CMP) processes. Assessing the toxicity of NPs used in commercial CMP slurries is difficult because these mixtures may contain undefined toxic constituents. Herein, the fresh water flea Daphnia magna (D. magna) was used to assess the effects of four model CMP slurries that did not contain known toxic additives. In the acute toxicity assessments, the key findings were that c-SiO₂ slurry caused a modest increase in body size indicative of a hormetic stress response, that the Al₂O₃ slurry was toxic to D. magna with a calculated 96 h LC-50 of 1.1 mg mL-1, that the CeO₂ and Al₂O₃ slurries caused significant dose-dependent decreases in body size, and that NP dissolution was not responsible for these responses. In the chronic toxicity assessments, the key findings were that the c-SiO₂ slurry caused a modest increase in reproduction indicative of a hormetic stress response, that the Al₂O₃ slurry lead to a modest increase in morbidity and a significant decrease in body size, and that the CeO₂ and Al₂O₃ slurries caused dose-dependent decreases in reproductive output. The acute and chronic toxicity results demonstrate that different model CMP slurries exert distinct and unpredictable effects on D. magna morbidity, growth, and reproductive output. Especially important is that the CeO₂ and Al₂O₃ slurries reduced D. magna reproduction upon chronic exposure at low applied doses, which could have adverse consequences to aquatic ecosystems.Item A Carbon Nanotube-based Raman-imaging Immunoassay For Evaluating Tumor Targeting Ligands(Royal Society of Chemistry, 2014-04-16) Bajaj, Pooja; Mikoryak, Carole; Wang, Ruhung; Bushdiecker II, David K.; Memon, Pauras; Draper, Rockford K.; Dieckmann, Gregg R.; Pantano, Paul; Musselman, Inga H.; Pantano, Paul; Musselman, Inga H.Herein, we describe a versatile immunoassay that uses biotinylated single-walled carbon nanotubes (SWNTs) as a Raman label, avidin-biotin chemistry to link targeting ligands to the label, and confocal Raman microscopy to image whole cells. Using a breast tumor cell model, we demonstrate the usefulness of the method to assess membrane receptor/ligand systems by evaluating a monoclonal antibody, Her-66, known to target the Her2 receptors that are overexpressed on these cells. We present two-dimensional Raman images of the cellular distribution of the SWNT labels corresponding to the distribution of the Her2 receptors in different focal planes through the cell with validation of the method using immunofluorescence microscopy, demonstrating that the Her-66-SWNT complexes were targeted to Her2 cell receptors.;