Hsa-miR-1246, Hsa-miR-320a and Hsa-miR-196b-5p Inhibitors can Reduce the Cytotoxicity of Ebola Virus Glycoprotein in Vitro

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dc.contributor.ISNI0000 0001 1707 1372 (Zhang, MQ)
dc.contributor.LCNA99086074‏ (Zhang, MQ)en_US
dc.contributor.authorSheng, MiaoMiaoen_US
dc.contributor.authorYing, Zhongen_US
dc.contributor.authorYang,Chenen_US
dc.contributor.authorDu, JianChaoen_US
dc.contributor.authorJu, XiangWuen_US
dc.contributor.authorChen, Zhaoen_US
dc.contributor.authorGuiGen, Zhangen_US
dc.contributor.authorLiFang,Zhangen_US
dc.contributor.authorLiu, KangTaien_US
dc.contributor.authorYang, Ningen_US
dc.contributor.authorXie, Pengen_US
dc.contributor.authorLi, DangShengen_US
dc.contributor.authorZhang, Michael Q.en_US
dc.contributor.authorJiang, ChengYuen_US
dc.contributor.authorATLAS Collaborationen_US
dc.contributor.utdAuthorZhang, Michael Q.en_US
dc.date.accessioned2015-01-13T23:04:32Z
dc.date.available2015-01-13T23:04:32Z
dc.date.created2014-09-12en_US
dc.date.issued2014-09-12en_US
dc.descriptionIncludes supplementary material.en_US
dc.description.abstractEbola virus (EBOV) causes a highly lethal hemorrhagic fever syndrome in humans and has been associated with mortality rates of up to 91% in Zaire, the most lethal strain. Though the viral envelope glycoprotein (GP) mediates widespread inflammation and cellular damage, these changes have mainly focused on alterations at the protein level, the role of microRNAs (miRNAs) in the molecular pathogenesis underlying this lethal disease is not fully understood. Here, we report that the miRNAs hsa-miR-1246, hsa-miR-320a and hsa-miR-196b-5p were induced in human umbilical vein endothelial cells (HUVECs) following expression of EBOV GP. Among the proteins encoded by predicted targets of these miRNAs, the adhesion-related molecules tissue factor pathway inhibitor (TFPI), dystroglycan1 (DAG1) and the caspase 8 and FADD-like apoptosis regulator (CFLAR) were significantly downregulated in EBOV GP-expressing HUVECs. Moreover, inhibition of hsa-miR-1246, hsa-miR-320a and hsa-miR-196b-5p, or overexpression of TFPI, DAG1 and CFLAR rescued the cell viability that was induced by EBOV GP. Our results provide a novel molecular basis for EBOV pathogenesis and may contribute to the development of strategies to protect against future EBOV pandemics.en_US
dc.description.sponsorshipThis work was supported by the National Natural Science Foundation of China (81230002, 81300057, 91019016, 31361163004), National Basic Research Program of China (2012CB316503), Ministry of Health (201302017), Ministry of Science and Technology of China (2006AA02Z152), Program of Introducing Talents of Discipline to Universities (B08007) and Science and Technology Commission of Shanghai Municipality (07pj14096).en_US
dc.identifier.bibliographicCitationSheng, MiaoMiao, Zhong Ying, Chen Yang, JianChao Du, XiangWu Ju, et al. 2014. "Hsa-miR-1246, hsa-miR-320a and hsa-miR-196b-5p inhibitors can reduce the cytotoxicity of Ebola virus glycoprotein in vitro." Science China-Life Sciences 57(10): 959-972.en_US
dc.identifier.issn1674-7305en_US
dc.identifier.issue10en_US
dc.identifier.urihttp://hdl.handle.net/10735.1/4262
dc.identifier.volume57en_US
dc.language.isoenen_US
dc.publisherScience Pressen_US
dc.relation.urihttp://dx.doi.org/10.1007/s11427-014-4742-y
dc.rightsCC BY 4.0 (Attribution)en_US
dc.rights©2014 CERN for the ATLAS Collaborationen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourceScience China-Life Sciences
dc.subjectMIRN1246 microRNA, humanen_US
dc.subjectMIRN320 microRNA, humanen_US
dc.subjectMIRN196 microRNA, humanen_US
dc.subjectEbola virus diseaseen_US
dc.subjectGlycoproteinsen_US
dc.subjectCell-mediated cytotoxicityen_US
dc.titleHsa-miR-1246, Hsa-miR-320a and Hsa-miR-196b-5p Inhibitors can Reduce the Cytotoxicity of Ebola Virus Glycoprotein in Vitroen_US
dc.typetext
dc.type.genreArticleen_US

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Zhang, Michael Q.