Complete Genome Analysis of Three Acinetobacter Baumannii Clinical Isolates in China for Insight into the Diversification of Drug Resistance Elements

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dc.contributor.authorZhu, Lingxiangen_US
dc.contributor.authorYan, Zhongqiangen_US
dc.contributor.authorZhang, Zhaojunen_US
dc.contributor.authorZhou, Qimingen_US
dc.contributor.authorZhou, Jinchunen_US
dc.contributor.authorWakeland, Edward K.en_US
dc.contributor.authorFang, Xiangdongen_US
dc.contributor.authorXuan, Zhenyuen_US
dc.contributor.authorShen, Dingxiaen_US
dc.contributor.authorLi, Quan-Zhenen_US
dc.contributor.utdAuthorXuan, Zhenyuen_US
dc.date.accessioned2014-09-03T20:50:59Z
dc.date.available2014-09-03T20:50:59Z
dc.date.created2013-06-24en_US
dc.date.issued2013-06-24en_US
dc.description.abstractBackground: The emergence and rapid spreading of multidrug-resistant Acinetobacter baumannii strains has become a major health threat worldwide. To better understand the genetic recombination related with the acquisition of drug-resistant elements during bacterial infection, we performed complete genome analysis on three newly isolated multidrug-resistant A. baumannii strains from Beijing using next-generation sequencing technology. Methodologies/Principal Findings: Whole genome comparison revealed that all 3 strains share some common drug resistant elements including carbapenem-resistant blaOXA₂₃ and tetracycline (tet) resistance islands, but the genome structures are diversified among strains. Various genomic islands intersperse on the genome with transposons and insertions, reflecting the recombination flexibility during the acquisition of the resistant elements. The blood-isolated BJAB07104 and ascites-isolated BJAB0868 exhibit high similarity on their genome structure with most of the global clone II strains, suggesting these two strains belong to the dominant outbreak strains prevalent worldwide. A large resistance island (RI) of about 121-kb, carrying a cluster of resistance-related genes, was inserted into the ATPase gene on BJAB07104 and BJAB0868 genomes. A 78-kb insertion element carrying tra-locus and blaOXA₂₃ island, can be either inserted into one of the tniB gene in the 121-kb RI on the chromosome, or transformed to conjugative plasmid in the two BJAB strains. The third strains of this study, BJAB0715, which was isolated from spinal fluid, exhibit much more divergence compared with above two strains. It harbors multiple drug-resistance elements including a truncated AbaR-22-like RI on its genome. One of the unique features of this strain is that it carries both blaOXA-23 and blaOXA-58 genes on its genome. Besides, an Acinetobacter lwoffii adeABC efflux element was found inserted into the ATPase position in BJAB0715. Conclusions: Our comparative analysis on currently completed Acinetobacter baumannii genomes revealed extensive and dynamic genome organizations, which may facilitate the bacteria to acquire drug-resistance elements into their genomes.en_US
dc.description.sponsorshipCapital Medical Development Research Fund, Beijing, China (No. 2009-1023); NIH (P50 CORT AR0558503, R03 AR055778).en_US
dc.identifier.bibliographicCitationZhu, Lingxiang, Zhongqiang Yan, Zhaojun Zhang, Qiming Zhou, et al. 2013. "Complete Genome Analysis of Three Acinetobacter baumannii Clinical Isolates in China for Insight into the Diversification of Drug Resistance Elements." PLOS One 8(6): e66584.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.issue6en_US
dc.identifier.urihttp://hdl.handle.net/10735.1/3953
dc.identifier.volume8en_US
dc.relation.urihttp://dx.doi.org/10.1371/journal.pone.0066584
dc.rightsCC BY 3.0 (Attribution)en_US
dc.rights©2013 The Authorsen_US
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/en_US
dc.sourcePLOS One
dc.subjectAcinetobacter baumanniien_US
dc.subjectChromosomesen_US
dc.subjectGenesen_US
dc.subjectBacterial genomesen_US
dc.subjectDrug resistance in microorganismsen_US
dc.titleComplete Genome Analysis of Three Acinetobacter Baumannii Clinical Isolates in China for Insight into the Diversification of Drug Resistance Elementsen_US
dc.type.genreArticleen_US

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