Heme Promotes Transcriptional and Demethylase Activities of Gis1, a Member of the Histone Demethylase JMJD2/KDM4 Family
dc.contributor.author | Lal, Sneha | en_US |
dc.contributor.author | Comer, Jonathan M. | en_US |
dc.contributor.author | Konduri, Purna C. | en_US |
dc.contributor.author | Shah, Ajit | en_US |
dc.contributor.author | Wang, Tianyuan | en_US |
dc.contributor.author | Lewis, Anthony | en_US |
dc.contributor.author | Shoffner, Grant | en_US |
dc.contributor.author | Guo, Feng | en_US |
dc.contributor.author | Zhang, Li | en_US |
dc.contributor.utdAuthor | Lal, Sneha | en_US |
dc.contributor.utdAuthor | Comer, Jonathan M. | en_US |
dc.contributor.utdAuthor | Konduri, Purna C. | en_US |
dc.contributor.utdAuthor | Wang, Tianyuan | en_US |
dc.contributor.utdAuthor | Lewis, Anthony | en_US |
dc.contributor.utdAuthor | Zhang, Li | en_US |
dc.date.accessioned | 2018-10-22T19:31:27Z | |
dc.date.available | 2018-10-22T19:31:27Z | |
dc.date.created | 2017-11-06 | en_US |
dc.date.issued | 2018-10-22 | |
dc.description.abstract | The yeast Gis1 protein is a transcriptional regulator belonging to the JMJD2/KDM4 subfamily of demethylases that contain a JmjC domain, which are highly conserved from yeast to humans. They have important functions in histone methylation, cellular signaling and tumorigenesis. Besides serving as a cofactor in many proteins, heme is known to directly regulate the activities of proteins ranging from transcriptional regulators to potassium channels. Here, we report a novel mechanism governing heme regulation of Gis1 transcriptional and histone demethylase activities. We found that two Gis1 modules, the JmjN + JmjC domain and the zinc finger (ZnF), can bind to heme specifically in vitro. In vivo functional analysis showed that the ZnF, not the JmjN + JmjC domain, promotes heme activation of transcriptional activity. Likewise, measurements of the demethylase activity of purified Gis1 proteins showed that full-length Gis1 and the JmjN + JmjC domain both possess demethylase activity. However, heme potentiates the demethylase activity of full-length Gis1, but not that of the JmjN + JmjC domain, which can confer heme activation of transcriptional activity in an unrelated protein. These results demonstrate that Gis1 represents a novel class of multi-functional heme sensing and signaling proteins, and that heme binding to the ZnF stimulates Gis1 demethylase and transcriptional activities. | en_US |
dc.description.department | School of Natural Sciences and Mathematics | en_US |
dc.description.sponsorship | National Institute of General Medical Sciences [R15GM107717]. Funding for open access charge: National Institute of General Medical Sciences [R15GM107717]. | en_US |
dc.identifier.bibliographicCitation | Lal, Sneha, Jonathan M. Comer, Purna C. Konduri, Ajit Shah, et al. 2018. "Heme promotes transcriptional and demethylase activities of Gis1, a member of the histone demethylase JMJD2/KDM4 family" Nucleic Acids Research 46(1), doi:10.1093/nar/gkx1051 | en_US |
dc.identifier.issn | 1362-4962 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.uri | http://hdl.handle.net/10735.1/6214 | |
dc.identifier.volume | 46 | en_US |
dc.language.iso | en | en_US |
dc.publisher | Oxford University Press | en_US |
dc.relation.uri | http://dx.doi.org/10.1093/nar/gkx1051 | |
dc.rights | CC BY 4.0 (Attribution) | en_US |
dc.rights | ©2017 The Authors | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.source | Nucleic Acids Research | |
dc.subject | GIS1 protein, S cerevisiae | en_US |
dc.subject | Histone Demethylases | en_US |
dc.subject | Jumonji C domain | en_US |
dc.subject | Jumonji Domain-Containing Histone Demethylases | en_US |
dc.title | Heme Promotes Transcriptional and Demethylase Activities of Gis1, a Member of the Histone Demethylase JMJD2/KDM4 Family | en_US |
dc.type.genre | article | en_US |
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