dc.contributor.author	de Solis, Christopher A.	en_US
dc.contributor.author	Morales, Anna A.	en_US
dc.contributor.author	Hosek, Matthew P.	en_US
dc.contributor.author	Partin, Alex C.	en_US
dc.contributor.author	Ploski, Jonathan E.	en_US
dc.contributor.utdAuthor	de Solis, Christopher A.	en_US
dc.contributor.utdAuthor	Morales, Anna A.	en_US
dc.contributor.utdAuthor	Hosek, Matthew P.	en_US
dc.contributor.utdAuthor	Ploski, Jonathan E.	en_US
dc.date.accessioned	2018-08-20T16:20:39Z
dc.date.available	2018-08-20T16:20:39Z
dc.date.created	2017-10-10
dc.description	Includes supplementary material	en_US
dc.description.abstract	There have been several attempts to identify which RNAs are localized to dendrites; however, no study has determined which RNAs localize to the dendrites following the induction of synaptic activity. We sought to identify all RNA transcripts that localize to the distal dendrites of dentate gyrus granule cells following unilateral high frequency stimulation of the perforant pathway (pp-HFS) using Sprague Dawley rats. We then utilized laser microdissection (LMD) to very accurately dissect out the distal 2/3rds of the molecular layer (ML), which contains these dendrites, without contamination from the granule cell layer, 2 and 4 h post pp-HFS. Next, we purified and amplified RNA from the ML and performed an unbiased screen for 27,000 RNA transcripts using Affymetrix microarrays. We determined that Activity Regulated Cytoskeletal Protein (Arc/Arg3.1) mRNA, exhibited the greatest fold increase in the ML at both timepoints 2 and 4 h). In total, we identified 31 transcripts that increased their levels within the ML following pp-HFS across the two timepoints. Of particular interest is that one of these identified transcripts was an unprocessed micro-RNA pri-miR132). Fluorescent in situ hybridization and qRT-PCR were used to confirm some of these candidate transcripts. Our data indicate Arc is a unique activity dependent gene, due to the magnitude that its activity dependent transcript localizes to the dendrites. Our study determined other activity dependent transcripts likely localize to the dendrites following neural activity, but do so with lower efficiency compared to Arc.	en_US
dc.description.department	School of Behavioral and Brain Sciences	en_US
dc.description.sponsorship	NIH grant RMH109945	en_US
dc.identifier.bibliographicCitation	de Solis, Christopher A., Anna A. Morales, Matthew P. Hosek, Alex C. Partin, et al. 2017. "Is Arc mRNA Unique: A Search for mRNAs That Localize to the Distal Dendrites of Dentate Gyrus Granule Cells Following Neural Activity." Frontiers in Molecular Neuroscience 10(314), doi: 10.3389/fnmol.2017.00314	en_US
dc.identifier.issn	1662-5099	en_US
dc.identifier.uri	http://hdl.handle.net/10735.1/5984
dc.identifier.volume	10	en_US
dc.language.iso	en	en_US
dc.publisher	Frontiers Media Sa	en_US
dc.relation.uri	http://dx.doi.org/10.3389/fnmol.2017.00314	en_US
dc.rights	CC BY 4.0 (Attribution)	en_US
dc.rights	©2017 The Authors. All Rights Reserved.	en_US
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	en_US
dc.source.journal	Frontiers In Molecular Neuroscience	en_US
dc.subject	MicroRNAs	en_US
dc.subject	Genes, Immediate-Early	en_US
dc.subject	Amygdala	en_US
dc.subject	Memory	en_US
dc.subject	Rats, Sprague-Dawley	en_US
dc.subject	Dendrites	en_US
dc.subject	Hippocampus	en_US
dc.subject	Perforant Pathway	en_US
dc.subject	Dentate Gyrus	en_US
dc.title	Is Arc mRNA Unique: A Search For mRNAs That Localize to the Distal Dendrites of Dentate Gyrus Granule Cells Following Neural Activity	en_US
dc.type	Text	en_US
dc.type.genre	article	en_US

Is Arc mRNA Unique: A Search For mRNAs That Localize to the Distal Dendrites of Dentate Gyrus Granule Cells Following Neural Activity

Files

Original bundle

Collections