The Nuclear Localization of SWI/SNF Proteins Is Subjected to Oxygen Regulation

Simple item page
dc.contributor.authorDastidar, Ranita Ghoshen_US
dc.contributor.authorHooda, Jagmohanen_US
dc.contributor.authorShah, Ajiten_US
dc.contributor.authorCao, Thai M.en_US
dc.contributor.authorRobert Michael Henkeen_US
dc.contributor.authorZhang, Lien_US
dc.contributor.utdAuthorZhang, Lien_US
dc.date.accessioned2014-02-28T21:32:30Z
dc.date.available2014-02-28T21:32:30Z
dc.date.created2012-07-13en_US
dc.date.issued2012-08-29en_US
dc.description.abstractBackground: Hypoxia is associated with many disease conditions in humans, such as cancer, stroke and traumatic injuries. Hypoxia elicits broad molecular and cellular changes in diverse eukaryotes. Our recent studies suggest that one likely mechanism mediating such broad changes is through changes in the cellular localization of important regulatory proteins. Particularly, we have found that over 120 nuclear proteins with important functions ranging from transcriptional regulation to RNA processing exhibit altered cellular locations under hypoxia. In this report, we describe further experiments to identify and evaluate the role of nuclear protein relocalization in mediating hypoxia responses in yeast.Results: To identify regulatory proteins that play a causal role in mediating hypoxia responses, we characterized the time courses of relocalization of hypoxia-altered nuclear proteins in response to hypoxia and reoxygenation. We found that 17 nuclear proteins relocalized in a significantly shorter time period in response to both hypoxia and reoxygenation. Particularly, several components of the SWI/SNF complex were fast responders, and analysis of gene expression data show that many targets of the SWI/SNF proteins are oxygen regulated. Furthermore, confocal fluorescent live cell imaging showed that over 95% of hypoxia-altered SWI/SNF proteins accumulated in the cytosol in hypoxic cells, while over 95% of the proteins were nuclear in normoxic cells, as expected.Conclusions: SWI/SNF proteins relocalize in response to hypoxia and reoxygenation in a quick manner, and their relocalization likely accounts for, in part or in whole, oxygen regulation of many SWI/SNF target genes.en_US
dc.description.sponsorshipFunding provided by grants from NIH (GM62246) and NCI Cancer Center (1P30 CA142543-01).
dc.identifier.bibliographicCitationDastidar, R. G., J. Hooda, A. Shah, T. M. Cao, et al. 2012. "The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation." Cell and Bioscience 2(1).en_US
dc.identifier.issn2045-3701en_US
dc.identifier.issue1en_US
dc.identifier.urihttp://hdl.handle.net/10735.1/3126
dc.identifier.volume2en_US
dc.relation.urihttp://dx.doi.org/10.1186/2045-3701-2-30
dc.rightsCC BY 2.0 (Attribution)en_US
dc.rights© 2012 Ghosh Dastidar et al.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/en_US
dc.sourceCell and Bioscience
dc.subjectCell Hypoxiaen_US
dc.subjectGene expressionen_US
dc.subjectOxygen-regulated Proteinsen_US
dc.subjectProtein Transporten_US
dc.subjectSWI/SNFen_US
dc.titleThe Nuclear Localization of SWI/SNF Proteins Is Subjected to Oxygen Regulationen_US
dc.typeTexten_US
dc.type.genreArticleen_US

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
NSM-FR-LiZhang-310502.92.pdf
Size:
2.45 MB
Format:
Adobe Portable Document Format
Download

Collections

Zhang, Li