DNA Looping in Topologically Constrained Domains
Date
2012-05-15
ORCID
Journal Title
Journal ISSN
Volume Title
Publisher
Oxford University Press.
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Abstract
New techniques are needed in order to study the effects of DNA supercoiling on loop-mediated regulation. The thermodynamics of DNA looping depend periodically on the helical phasing between recognition sites. A long-term goal is to provide enabling technologies for ensemble and single-molecule FRET studies of DNA looping mediated by lac repressor in vitro and in vivo. Other applications of the methodology include engineering of DNA substrates for studies of site-specific recombination and other enzyme systems
Description
Keywords
DNA, Fluorescence resonance energy transfer, Recombination, genetic, DNA-protein interactions
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This work was supported by grants from the DMS/NIGMS Joint
Program in Mathematical Biology (GM67242 and DMS-800929).
Rights
CC BY-NC 3.0 (Attribution-NonCommercial), ©2012 The Authors